Anti-Mycobacterial 30-kDa [3A8]
Invented by Professor Pranab K Das
Invented at
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- References (8)
- Inventor Info
Info
| Catalogue Number | 154075 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Mycobacterial 30-kDa |
| Reactivity | Mycobacterium bovis |
| Relevance | Proteins of the antigen 85 complex in the 30-kDa region secreted by live mycobacteria are important in the immune response against mycobacterial infections and play an important biological role in the host-parasite interaction. This antibody recognises antigens 85A (MPT44), 85B (MPT59) and 85C (MPT45). This antibody stains the paucibacillary borderline and tuberculoid leprosy lesions. |
| Host | Mouse |
| Immunogen | 30-kDa antigen isolated from M.tuberculosis (RIVM-strain 7114) |
| Positive Control | M.tuberculosis |
| Subclass | IgG1 |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | RPMI 1640 |
| Strain | Balb/c |
| Notes | Reacts with M.bovis BCG, M.tuberculosis complex and M.leprae. Does not react with E.coli. The 3A8 epitope has been shown to be present on CD68+ macrophages in the granulomas of tuberculoid leprosy. In lesions from reversal reaction the 3A8 epitope is more strongly expressed on CD1a+ dendritic Langerhans cells both in the epidermis and dermis. |
| Research Area | Bacteriology, Immunology, Microbiology |
References: 8 entries
Rambukkana et al. 1993. Infect Immun. 61(5):1835-45. PMID: 7682995.
The mycobacterial secreted antigen 85 complex possesses epitopes that are differentially expressed in human leprosy lesions and Mycobacterium leprae-infected armadillo tissues.
Europe PMC ID: 7682995
Rambukkana et al. 1992. Infect Immun. 60(12):5172-81. PMID: 1280626.
Heterogeneity of monoclonal antibody-reactive epitopes on mycobacterial 30-kilodalton-region proteins and the secreted antigen 85 complex and demonstration of antigen 85B on the Mycobacterium leprae cell wall surface.
Europe PMC ID: 1280626
Rambukkana et al. 1992. Scand J Immunol. 36(1):35-48. PMID: 1615282.
Association of the mycobacterial 30-kDa region proteins with the cutaneous infiltrates of leprosy lesions. Evidence for the involvement of the major mycobacterial secreted proteins in the local immune response of leprosy.
Europe PMC ID: 1615282
Rambukkana et al. 1991. Scand J Immunol. 33(6):763-75. PMID: 1904624.
Subcellular distribution of monoclonal antibody defined epitopes on immunodominant Mycobacterium tuberculosis proteins in the 30-kDa region: identification and localization of 29/33-kDa doublet proteins on mycobacterial cell wall.
Europe PMC ID: 1904624
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References: 8 entries
Rambukkana et al. 1993. Infect Immun. 61(5):1835-45. PMID: 7682995.
The mycobacterial secreted antigen 85 complex possesses epitopes that are differentially expressed in human leprosy lesions and Mycobacterium leprae-infected armadillo tissues.
Rambukkana et al. 1992. Infect Immun. 60(12):5172-81. PMID: 1280626.
Heterogeneity of monoclonal antibody-reactive epitopes on mycobacterial 30-kilodalton-region proteins and the secreted antigen 85 complex and demonstration of antigen 85B on the Mycobacterium leprae cell wall surface.
Rambukkana et al. 1992. Scand J Immunol. 36(1):35-48. PMID: 1615282.
Association of the mycobacterial 30-kDa region proteins with the cutaneous infiltrates of leprosy lesions. Evidence for the involvement of the major mycobacterial secreted proteins in the local immune response of leprosy.
Rambukkana et al. 1991. Scand J Immunol. 33(6):763-75. PMID: 1904624.
Subcellular distribution of monoclonal antibody defined epitopes on immunodominant Mycobacterium tuberculosis proteins in the 30-kDa region: identification and localization of 29/33-kDa doublet proteins on mycobacterial cell wall.
Add a reference
