J558L Cell Line secreting OX40L
Invented at University Of Birmingham
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153661 |
| Antigen/Gene or Protein Targets | Murine OX40L |
| Parental Line | J558L - mammalian plasmacytoma cell line |
| Synonyms | OX40L ligand, CD134, dendritic cells, DCs, Th2, CD525, systemic lupus erythematosus, TXGP1, OX-40L, gp34, TNFSF4, CD4 T cells, cytokine, Tumor necrosis factor receptor superfamily member 4, TNFRSF4 |
| Host | Mouse |
| Relevance | OX40 receptor also known as CD134, a member of the tumor necrosis factor receptor superfamily of receptors, is not constitutively expressed on resting naive T cells. OX40 is activated 24 to 72 hours after binding to its ligand; OX40L, and is a secondary co-stimulatory immune checkpoint molecule. Expression of OX40 is dependent on full activation of the CD28 absent-T cell. |
| Production Details | Primers specific to the murine sequence of the OX40L gene were used to generate a cDNA amplicon which was cloned into an expression vector. The vector has resistant markers to ampicillin and histidinol, and also contains promoters and enhancers for murine B-cells and plasma cells. A chimeric IgG1 and OX40 molecule with a 3' splice donor site was generated by subcloning the OX40L amplicon into a human IgG1 expression vector. |
| Conditional | No |
| Research Area | Immunology |
References: 2 entries
Flynn et al. 1998. J Exp Med. 188(2):297-304. PMID: 9670042.
CD4 T cell cytokine differentiation: the B cell activation molecule, OX40 ligand, instructs CD4 T cells to express interleukin 4 and upregulates expression of the chemokine receptor, Blr-1.
Europe PMC ID: 9670042
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References: 2 entries
Flynn et al. 1998. J Exp Med. 188(2):297-304. PMID: 9670042.
CD4 T cell cytokine differentiation: the B cell activation molecule, OX40 ligand, instructs CD4 T cells to express interleukin 4 and upregulates expression of the chemokine receptor, Blr-1.
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