UB-UE1 Cell Line
Invented by Matthew C Holley
Invented at University Of Bristol , University Of Sheffield
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 153624 |
| Parental Line | pure utricular sensory epithelia of 2-day old immortomouse pups |
| Synonyms | Utricular Epithelium cell line number 1 |
| Host | Mouse |
| Model | Immortalised Line |
| Relevance |
Derived from vestibular epithelium (utricular macula) using thermolysin treatment and dissection to ensure origin from sensory epithelial supporting cells at post-natal day P2. At this stage the supporting cells are normally post-mitotic but retain the ability to differentiate as sensory hair cells and or supporting cells. UB/UE-1 was characterised by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for epithelial cells and for inner ear sensory cells. These include Cytokeratin, Vimentin, Myosin VIIa, Myosin VI, Brn3c, α9AChR and a combination of functional ion channels (see references). Screened with Affymetrix mouse Micro-arrays. The cells differentiate neonatal stage hair cell and supporting cell phenotypes under differentiating conditions in vitro. Epithelial cell line suitable for studies on: Inner ear development Gene expression and function of inner ear-specific genes In vitro screening for gene activation and promoter analysis Ototoxicity (prescribed drugs and agents that ameliorate their affects) Function of inherited deafness mutations Functional analysis of ion channels, receptors and signalling pathways in vitro |
| Conditional | Yes |
| Conditional Description | Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the γ–interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the Immortomouse™ (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100). |
| Research Area | Developmental Biology, Drug Discovery & Development, Gene Expression |
| Recommended Growing Conditions | MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine, 33°C |
References: 4 entries
Hackett et al. 2002. Exp Cell Res. 278(1):19-30. PMID: 12126954.
E-cadherin and the differentiation of mammalian vestibular hair cells.
Europe PMC ID: 12126954
Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448.
Differentiation of mammalian vestibular hair cells from conditionally immortal, postnatal supporting cells.
Europe PMC ID: 10531448
Add a reference
References: 4 entries
Hackett et al. 2002. Exp Cell Res. 278(1):19-30. PMID: 12126954.
E-cadherin and the differentiation of mammalian vestibular hair cells.
Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448.
Differentiation of mammalian vestibular hair cells from conditionally immortal, postnatal supporting cells.
Add a reference
Inventor Information
Inventors
|
Matthew C Holley |
