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|Applications||ChIP ELISA IHC IF IP WB|
|Antigen/Gene or Protein Targets||c-Myc|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is N-EQKLISEEDL-C, where N stands for Amino-terminus and C stands for Carboxy terminus. The tag is approximately 1202 Daltons in atomic mass and has 10 amino acids.
It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
Monoclonal 5A5 was selected by its excellent performance in ChIP analyses.
|Immunogen||Synthetic peptide: EQKLISEEDL conjugated to KLH, corresponding to C terminal amino acids 408-432 of Human c-Myc.|
|Molecular Weight (kDa)||1.2|
|Notes||Epitope aa 410-419 of human c-Myc|
|Research Area||Cancer, Cell Cycle, Epitope Tags, Gene Expression|
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