![Cyclin B1-Venus Reporter Cell line [RPE1 cycB1-venus/+ KI clone 20G11] Metaphase, example 1](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/7acd79a2-1091-4168-8dcf-1f83ae2cb8f2.png)
![Cyclin B1-Venus Reporter Cell line [RPE1 cycB1-venus/+ KI clone 20G11] Metaphase, example 2](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/890a02af-a47b-4694-a343-feebd31a65a3.png)
![Cyclin B1-Venus Reporter Cell line [RPE1 cycB1-venus/+ KI clone 20G11] Metaphase, example 1](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/7acd79a2-1091-4168-8dcf-1f83ae2cb8f2.png)
![Cyclin B1-Venus Reporter Cell line [RPE1 cycB1-venus/+ KI clone 20G11] Metaphase, example 2](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/890a02af-a47b-4694-a343-feebd31a65a3.png)
![Image thumbnail for Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/7acd79a2-1091-4168-8dcf-1f83ae2cb8f2.png)
![Image thumbnail for Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/890a02af-a47b-4694-a343-feebd31a65a3.png)
Cyclin A2-Venus Reporter Cell Line [RPE1 cycA2-venus/+ KI clone D6]
Invented by Dr Jonathon Pines at University of Cambridge
![Cyclin A2-Venus Reporter Cell line [RPE1 cycA2-venus/+ KI clone D6]](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/7ed9a86c-9662-4194-b1c2-e98387a5c23e.png)
Cyclin B1-Venus Reporter Cell Line [RPE1 cycB1-venus/+ KI clone 20G11]
Invented by Dr Jonathon Pines from Gurdon Institute
Invented at University of Cambridge
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153464 |
| Antigen/Gene or Protein Targets | Cyclin B1 |
| Parental Line | RPE1 |
| Synonyms | CCNB1 |
| Host | Human |
| Tissue | Eye |
| Model | Reporter |
| Relevance | Somatic knock-in reporter cell with endogenous expression of cyclin B1 fused to the yellow fluorescent protein Venus (cyclin B1-Venus fusion protein). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This Cyclin B1 cell line functions as a cell cycle reporter across the G2/mitotic-specific phase of the cell cycle. See also the Mad2 and Cyclin A2 versions of these cell lines for a broader analysis of the different phases of the cell cycle. This cell line is an endogenous reporter for cyclin B1 levels and activity. |
| Production Details | To generate the cell line recombinant adenovirus-associated virus (rAAV)-mediated gene targeting was used to fuse the open reading frame (ORF) of yellow fluorescent protein (Venus) with exon 9 of the CCNB1 (Cyclin B1) gene in hTert-RPE1 cells (RPE1; retinal pigment epithelial). RPE1 cells were chosen because they have a normal diploid karyotype, are not transformed and exhibit little cell death when arrested in mitosis; tagging the endogenous Cyclin B1 protein in RPE1 cells avoided the complications of mosaic protein levels in the cell population produced by ectopic expression. |
| Conditional | No |
| Research Area | Cell Cycle |
| Recommended Growing Conditions | F12/DMEM (Sigma: D6241) + 2mM GlutaMAX(Invitrogen), 10% Foetal Bovine Serum (FBS) + 0.348% sodium bicarbonate; 37oC; 5% CO2. |
![Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1] Metaphase](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/d403407e-097f-4c1e-9795-64bd69ec8ac3.png)
References: 2 entries
Collin et al. 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242.
The spindle assembly checkpoint works like a rheostat rather than a toggle switch.
Europe PMC ID: 24096242
Add a reference
References: 2 entries
Collin et al. 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242.
The spindle assembly checkpoint works like a rheostat rather than a toggle switch.
Add a reference
