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|Antigen/Gene or Protein Targets||This model encodes a triple targeted mutation with disruption of the multi-drug resistance genes Abcb1a, Abcb1b and Abcg2.|
|Synonyms||p-glycoprotein 1, permeability glycoprotein 1, P-gp, pgp, MDR1, ABCB1, CD243 p-glycoprotein 3, BCRP/ABCG2, ATP-binding cassette sub-family G member 2, CDw338,|
P-gp, a member of the MDR/TAP subfamily, is a glycoprotein encoded in humans by the ABCB1 gene. P-gp is a well-characterized ABC-transporter responsible for transporting a wide variety of substrates across extra- and intracellular membranes.
The normal excretion of xenobiotics back into the gut lumen by P-gp pharmacokinetically reduces the efficacy of some pharmaceutical drugs and in addition, some cancer cells also express large amounts of P-gp which can further enhance that effect. This makes some cancers multi-drug resistant.
|Production Details||This model was generated by breeding the Mdr1a/b mutated mouse with the Bcrp mutated mouse. The Mdr1a/b model was created by sequential targeting of the two Abcb1a and Abcb1b genes in E14 ES cells. Resultant chimeras were backcrossed to FVB/N for seven generations. The Bcrp model was created by targeting the Abcg2 gene in E14 embryonic stem cells derived from 129P2/OlaHsd mice and injecting the targeted cells into FVB blastocysts.|
|Mouse Genetic Background/Cross History||FVB Background|
Homozygous for all three mutations.
Wild type for Nnt mutation; carries Pde6brd1 mutation
|Research Area||Cancer, Drug Discovery & Development|
The Mdr1a/b-Bcrp mouse was developed in the laboratory of Alfred Schinkel of the Netherlands Cancer Institute.
Useful in studies of drug transport, oral bioavailability and multi-drug resistance
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