HR1 Cell Line
Invented by Dr Simon Cook at Babraham Institute
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 153328 |
| Antigen/Gene or Protein Targets | MEKK3 |
| Parental Line | HEK 293 |
| Synonyms | Mitogen-activated protein kinase kinase kinase 3, MAPK/ERK kinase kinase 3, MEK kinase 3, MEKK 3 |
| Host | Human |
| Tissue | Kidney |
| Disease Keywords | Cell signalling pathways; cancer; inflammation |
| Relevance |
HM3 cells are HEK 293 cells stably expressing conditional kinase ∆MEKK3:ER* from the pBabePuro plasmid. ∆MEKK3:ER* consists of the isolated kinase domain of MEKK3 fused in-frame to a modified form of the hormone binding domain of the estrogen receptor (hbER*) that can be de-repressed by 4-hydroxytamoxifen (4-HT) but not β-estradiol. In this case the * refers to a point mutation that ablates estradiol binding but allows 4-HT binding. Activation of ∆MEKK3:ER* leads to the strong activation of the JNK and p38 pathways and a weaker activation of ERK1/2. This cell line can be used to study the role and factors impacting on the JNK, p38 and ERK1/2 signalling pathways such as gene expression, cell proliferation, cell cycle arrest and cell death.. The cells are puromycin resistant. Conditional kinase activation of ∆MEKK3:ER* can be induced with 100nM 4-HT. |
| Production Details | HEK 293 cells were transfected with the pBabePuro plasmid expressing ∆MEKK3:ER*. Stable transfectants were selected using puromycin and ring cloning. |
| Conditional | Yes |
| Conditional Description | Stable transfectant, conditional functionality: MEKK3 kinase activity and ERK1/2, JNK and p38 signalling pathways activated following 4-hydroxytamoxifen (4-HT) treatment of cells. |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, Cell Signaling & Signal Transduction, Immunology |
| Growth/Phenotype Keywords | Adherent cell line |
| Recommended Growing Conditions | Phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) high glucose version, 2 mM L-glutamine, 10% fetal bovine serum (FBS), 2 µg/ml puromycin. |
| Cellosaurus ID | CVCL_0306 |
References: 6 entries
Gilley et al. 2009. Cell Signal. 21(6):969-77. PMID: 19249353.
ERK1/2, but not ERK5, is necessary and sufficient for phosphorylation and activation of c-Fos.
Europe PMC ID: 19249353
Boughan et al. 2006. J Biol Chem. 281(17):11637-48. PMID: 16513653.
Nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor: critical regulators of beta-defensins during Helicobacter pylori infection.
Europe PMC ID: 16513653
Todd et al. 2004. Oncogene. 23(19):3284-95. PMID: 14981547.
ERK1/2 and p38 cooperate to induce a p21CIP1-dependent G1 cell cycle arrest.
Europe PMC ID: 14981547
Add a reference
References: 6 entries
Gilley et al. 2009. Cell Signal. 21(6):969-77. PMID: 19249353.
ERK1/2, but not ERK5, is necessary and sufficient for phosphorylation and activation of c-Fos.
Boughan et al. 2006. J Biol Chem. 281(17):11637-48. PMID: 16513653.
Nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor: critical regulators of beta-defensins during Helicobacter pylori infection.
Todd et al. 2004. Oncogene. 23(19):3284-95. PMID: 14981547.
ERK1/2 and p38 cooperate to induce a p21CIP1-dependent G1 cell cycle arrest.
Add a reference
