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Anti-Mycobacterium avium subsp. paratuberculosis [6G11]

Invented by Dr Irene Grant at Queen's University Belfast, Dr Linda Stewart at Queen's University Belfast

Info

Catalogue Number 153318
Applications ELISA IP
Antigen/Gene or Protein Targets Mycobacterium avium subsp. paratuberculosis
Reactivity Mycobacterium avium
Relevance Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent for Johne’s disease, a highly-infectious wasting disease that affects a range of domestic ruminants including cattle, sheep, goats and deer. Anti-Mycobacterium avium subsp. paratuberculosis [6G11] has a high degree of specificity and has been demonstrated to work by ELISA and for immunocapture.
Host Mouse
Immunogen Gamma-irradiated whole MAP strain B4 cells
Positive Control Mycobacterium avium subsp. paratuberculosis whole cells
Subclass IgM
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM containing 10% (v/v) fetal bovine serum and 5% (v/v) penicillin/streptomycin
Strain Balb/c
Notes Direct and competitive ELISA
Specificity of immunocapture was evaluated in O'Brien et al., 2016. The study assayed three non-target Mycobacterium spp. (M. avium subsp. avium, M. bovis BCG and M. terrae). Beads coated with 6G11, the bead combination MAb 6G11 and 15D10 (50:50 mix), and dually-coated MAb 6G11 and 54.3.1AA beads demonstrated a low percentage of non-specific capture (ranging from 0.75–15% capture, with M. bovis BCG giving the highest percentage capture for all bead types).
Research Area Bacteriology

References

There are 2 reference entries for this reagent.

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References: 2 entries

O'Brien et al. 2016. PLoS One. 11(1):e0147870. PMID: 26815790.

Novel Monoclonal Antibody and Peptide Binders for Mycobacterium avium subsp. paratuberculosis and Their Application for Magnetic Separation.

Europe PMC ID: 26815790


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References: 2 entries

O'Brien et al. 2016. PLoS One. 11(1):e0147870. PMID: 26815790.

Novel Monoclonal Antibody and Peptide Binders for Mycobacterium avium subsp. paratuberculosis and Their Application for Magnetic Separation.


Add a reference