Anti-IgD [Z56P1H5*G6]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
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![Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues. Positive membranous immunostaining is observed in infected trout kidney using Anti-IgD [Z56P1H5*G6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/60185f4e-42b9-4258-aded-82865f453700.png)
![Rainbow trout serum was separated under reducing conditions (4–15% SDS-PAGE) and subsequently probed with anti-IgD antibody [Z56P1H5*G6]. Data revealed two dominant secreted IgD isoforms, 165 and 100 kDa consistent with existing literature.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/9b15ed00-c85e-41f7-a824-8004e02c722e.png)
![Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues. Positive membranous immunostaining is observed in infected trout kidney using Anti-IgD [Z56P1H5*G6]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/60185f4e-42b9-4258-aded-82865f453700.png)
![Rainbow trout serum was separated under reducing conditions (4–15% SDS-PAGE) and subsequently probed with anti-IgD antibody [Z56P1H5*G6]. Data revealed two dominant secreted IgD isoforms, 165 and 100 kDa consistent with existing literature.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/9b15ed00-c85e-41f7-a824-8004e02c722e.png)
![Image thumbnail for Anti-IgD [Z56P1H5*G6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/188ce377-3be7-4e70-af10-7aeb85814fb0.jpg)
![Image thumbnail for Anti-IgD [Z56P1H5*G6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/b00114df-7767-4ed7-9166-950243277ad8.jpg)
![Image thumbnail for Anti-IgD [Z56P1H5*G6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/60185f4e-42b9-4258-aded-82865f453700.png)
![Image thumbnail for Anti-IgD [Z56P1H5*G6]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/9b15ed00-c85e-41f7-a824-8004e02c722e.png)
| Catalogue Number | 153312 |
| Applications | ELISA FACS IHC WB |
| Antigen/Gene or Protein Targets | Immunoglobulin D Heavy Constant Delta |
| Synonyms | IgD, Immunoglobulin D Heavy Constant Delta |
| Reactivity | Salmon, Rainbow Trout and Brown Trout |
| Relevance | Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for broodstock health testing. Moreover, our Anti-IgD antibody provides a useful tool to monitor vaccine performance in fish and will assist in the: development of future vaccines. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide. Peptide immunogen is 80% identical to IgD channel catfish (Ictalurus punctatus), 70% identical to Japanese halibut (Paralichthys olivaceus), 70% identical to Chinese perch (Siniperca chuatsi). Antibody potentially could cross-react with turbot (Scophthalmus maximus) 66% similarity to peptide immunogen. |
| Positive Control | ELISA, immunized fish sera; FACS, Peripheral blood lymphocyte, head kidney cells |
| Subclass | IgG1 |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes | Recommended Usage Conditions: ELISA neat; Western blot neat; IHC neat, antigen retrieval microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0; FACS 1/2 dilution |
| Research Area | Aquaculture |
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