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Catalogue Number 153246
Host Human
Tissue Breast
Disease Keywords Breast cancer
Model Immortalised Line
Relevance fR2 cells have been shown to express Muc-1, also known as HMFG-1 antigen, which is a mucin-like component of human milk fat globule membranes. They are positive for keratin 8 and 18, but negative for keratins 4, 6, 7, 10, 13, 14, 16, 17 and 19. Although fR2 is morphologically different from fR5, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumorigenic in nude mice.
Production Details The breast epithelial cell line fR2 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Adherent
Recommended Growing Conditions Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10µg/ml insulin + 5µg/ml hydrocortisone + 10% FBS
Notes STR-PCR Data:
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 11,13
D5S818: 12,13
D7S820: 8,12
THO1: 9.3
TPOX: 8
vWA: 20
Cellosaurus ID CVCL_2444

References

There are 2 reference entries for this reagent.

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References: 2 entries

Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.

Establishment and characterization of SV40-transformed human breast epithelial cell lines.

Europe PMC ID: 6279290


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References: 2 entries

Chang et al. 1982. Cancer Res. 42(5):2040-53. PMID: 6279290.

Establishment and characterization of SV40-transformed human breast epithelial cell lines.


Add a reference