T47D-E2J Cell Line
Invented by Prof Joy Burchell at King's College London
T47D-STn Cell Line
Invented by Prof Joy Burchell from King's College London , Prof Joyce Taylor-Papadimitriou from King's College London
Invented at King's College London
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153170 |
| Antigen/Gene or Protein Targets | N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 - ST6GalNAc-1 |
| Parental Line | T47D |
| Host | Human |
| Tissue | Breast |
| Disease Keywords | Breast Cancer |
| Relevance |
The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-STn, was created as a breast cancer model which expresses the Sialyl-Tn, STn, antigen. T47D-STn cells overexpress the enzymes C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein and glycosyltransferase, ST6GalNAc-I. ST6GalNAc-I which is found throughout the Golgi, can catalyse the transfer of sialic acid to N-acetylgalactosamine and is predominately limited to the gastrointestinal tract in normal adult tissues. Expression of ST6GalNAc-I in human cells promotes formation of the STn antigen which is a saccharide structure (N-acetylgalactosamine) linked to serine or threonine by a glycosidic bond. STn antigens are not usually found on healthy cell surfaces but often on human breast cancer cells. Together with wild-type T47D and T47D-E2J these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns. |
| Conditional | No |
| Research Area | Cancer, Drug Discovery & Development |
| Recommended Growing Conditions | DMEM 10% FCS, 500µg/ml G418, 300µg/ml hygromycin |
References: 2 entries
Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.
The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.
Europe PMC ID: 16319059
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References: 2 entries
Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.
The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.
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