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Anti-Erythrocyte [34-3C]

Info

Applications IF Fn
Antigen/Gene or Protein Targets Erythrocytes
Reactivity Mouse
Relevance Monoclonal antibody which binds mouse erythrocytes, inducing anaemia.

Background and Research Application
Anti-erythrocyte [34-3C] is a monoclonal autoantibody-secreting hybridoma established from unmanipulated autoimmune-prone NZB mice.

The antibody efficiently binds to Fc receptors on macrophages inducing anaemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity.
Host Mouse
Immunogen Mouse erythrocytes
Subclass IgG2a
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-erythrocyte (34-3C) only recognises antigenic determinants expressed on mouse RBC and not on other species of RBC.

Useful for establishing in vivo model of haemolytic anaemia.

Concentration
1mg/ml as standard
Research Area Immunology

References

There are 6 reference entries for this reagent.

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References: 6 entries

Akter et al. 2019. PLoS Pathog. 15(2):e1007599. PMID: 30811498.

Meinderts et al. 2017. Blood Adv. 1(14):875-886. PMID: 29296731.

Kuhn et al. 2017. Antioxid Redox Signal. 26(13):718-742. PMID: 27889956.

Morimoto et al. 2016. PLoS Negl Trop Dis. 10(3):e0004505. PMID: 26942577.

Shibata et al. 1990. Int Immunol. 2(12):1133-41. PMID: 2090198.

Fn

Monoclonal anti-erythrocyte autoantibodies derived from NZB mice cause autoimmune hemolytic anemia by two distinct pathogenic mechanisms.

Europe PMC ID: 2090198


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References: 6 entries

Akter et al. 2019. PLoS Pathog. 15(2):e1007599. PMID: 30811498.

Meinderts et al. 2017. Blood Adv. 1(14):875-886. PMID: 29296731.

Kuhn et al. 2017. Antioxid Redox Signal. 26(13):718-742. PMID: 27889956.

Morimoto et al. 2016. PLoS Negl Trop Dis. 10(3):e0004505. PMID: 26942577.

Shibata et al. 1990. Int Immunol. 2(12):1133-41. PMID: 2090198.

Fn

Monoclonal anti-erythrocyte autoantibodies derived from NZB mice cause autoimmune hemolytic anemia by two distinct pathogenic mechanisms.


Add a reference

Inventor Information

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