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Catalogue Number 152719
Backbone Size (bp) 5006
Bacterial Resistance Ampicillin
Vector Type pGEX-KG
Antigen/Gene or Protein Targets C3 ADP-ribosyltransferase
Relevance TAT-C3 consists of the Clostridium botulinum C3 exozyme (C3 ADP-ribosyltransferase) linked to an HIV TAT leader sequence that permits transduction of the protein across the plasma membrane. The cell penetrating moiety allows rapid and efficient transport through the plasma membrane. Once in the cytosol, the cell penetrating moiety is released, allowing C3 Transferase to freely diffuse intracellularly and inactive RhoA, RhoB, and RhoC, but not related GTPases such as Cdc42 or Rac1.

The Exoenzyme C3 Transferase from Clostridium botulinum is commonly used to selectively inactivate the GTPases RhoA, RhoB, and RhoC, both in vivo and in vitro. C3 Transferase inhibits Rho proteins by ADP-ribosylation on asparagine 41 in the effector binding domain of the GTPase.

TAT-C3 can be used to inactive Rho proteins in fibroblasts, neurons, epithelial, endothelial, and hematopoietic cells as well as other primary and immortalized cell lines.
Research Area Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility
Notes Purification protocol detailed in Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.

References: 14 entries

Rodrigues et al. 2014. Nat Commun. 5:5458. PMID: 25413277.

RHOA inactivation enhances Wnt signalling and promotes colorectal cancer.

Europe PMC ID: 25413277

Croft et al. 2011. Cell Res. 21(4):666-82. PMID: 21079653.

p53-mediated transcriptional regulation and activation of the actin cytoskeleton regulatory RhoC to LIMK2 signaling pathway promotes cell survival.

Europe PMC ID: 21079653

Hanley et al. 2010. Proc Natl Acad Sci U S A. 107(27):12145-50. PMID: 20566876.

Motorized RhoGAP myosin IXb (Myo9b) controls cell shape and motility.

Europe PMC ID: 20566876

Kumar et al. 2009. Mol Cell Biol. 29(15):4262-73. PMID: 19470758.

The transcriptional activity of Sox9 in chondrocytes is regulated by RhoA signaling and actin polymerization.

Europe PMC ID: 19470758

Busche et al. 2008. J Cell Sci. 121(Pt 7):1025-35. PMID: 18334560.

Epithelial cell-cell contacts regulate SRF-mediated transcription via Rac-actin-MAL signalling.

Europe PMC ID: 18334560

Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.

Purification of TAT-C3 exoenzyme.

Europe PMC ID: 16472655

Pollock et al. 2005. Cancer Res. 65(4):1244-50. PMID: 15735008.

Oncogenic K-RAS is required to maintain changes in cytoskeletal organization, adhesion, and motility in colon cancer cells.

Europe PMC ID: 15735008


Add a reference

References: 14 entries

Rodrigues et al. 2014. Nat Commun. 5:5458. PMID: 25413277.

RHOA inactivation enhances Wnt signalling and promotes colorectal cancer.

Europe PMC ID: 25413277

Croft et al. 2011. Cell Res. 21(4):666-82. PMID: 21079653.

p53-mediated transcriptional regulation and activation of the actin cytoskeleton regulatory RhoC to LIMK2 signaling pathway promotes cell survival.

Europe PMC ID: 21079653

Hanley et al. 2010. Proc Natl Acad Sci U S A. 107(27):12145-50. PMID: 20566876.

Motorized RhoGAP myosin IXb (Myo9b) controls cell shape and motility.

Europe PMC ID: 20566876

Kumar et al. 2009. Mol Cell Biol. 29(15):4262-73. PMID: 19470758.

The transcriptional activity of Sox9 in chondrocytes is regulated by RhoA signaling and actin polymerization.

Europe PMC ID: 19470758

Busche et al. 2008. J Cell Sci. 121(Pt 7):1025-35. PMID: 18334560.

Epithelial cell-cell contacts regulate SRF-mediated transcription via Rac-actin-MAL signalling.

Europe PMC ID: 18334560

Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.

Purification of TAT-C3 exoenzyme.

Europe PMC ID: 16472655

Pollock et al. 2005. Cancer Res. 65(4):1244-50. PMID: 15735008.

Oncogenic K-RAS is required to maintain changes in cytoskeletal organization, adhesion, and motility in colon cancer cells.

Europe PMC ID: 15735008


Add a reference

References: 14 entries

Rodrigues et al. 2014. Nat Commun. 5:5458. PMID: 25413277.

RHOA inactivation enhances Wnt signalling and promotes colorectal cancer.

Croft et al. 2011. Cell Res. 21(4):666-82. PMID: 21079653.

p53-mediated transcriptional regulation and activation of the actin cytoskeleton regulatory RhoC to LIMK2 signaling pathway promotes cell survival.

Hanley et al. 2010. Proc Natl Acad Sci U S A. 107(27):12145-50. PMID: 20566876.

Motorized RhoGAP myosin IXb (Myo9b) controls cell shape and motility.

Kumar et al. 2009. Mol Cell Biol. 29(15):4262-73. PMID: 19470758.

The transcriptional activity of Sox9 in chondrocytes is regulated by RhoA signaling and actin polymerization.

Busche et al. 2008. J Cell Sci. 121(Pt 7):1025-35. PMID: 18334560.

Epithelial cell-cell contacts regulate SRF-mediated transcription via Rac-actin-MAL signalling.

Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.

Purification of TAT-C3 exoenzyme.

Pollock et al. 2005. Cancer Res. 65(4):1244-50. PMID: 15735008.

Oncogenic K-RAS is required to maintain changes in cytoskeletal organization, adhesion, and motility in colon cancer cells.


Add a reference


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