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Catalogue Number 152719
Backbone Size (bp) 5006
Bacterial Resistance Ampicillin
Vector Type pGEX-KG
Antigen/Gene or Protein Targets C3 ADP-ribosyltransferase
Relevance TAT-C3 consists of the Clostridium botulinum C3 exozyme (C3 ADP-ribosyltransferase) linked to an HIV TAT leader sequence that permits transduction of the protein across the plasma membrane. The cell penetrating moiety allows rapid and efficient transport through the plasma membrane. Once in the cytosol, the cell penetrating moiety is released, allowing C3 Transferase to freely diffuse intracellularly and inactive RhoA, RhoB, and RhoC, but not related GTPases such as Cdc42 or Rac1.

The Exoenzyme C3 Transferase from Clostridium botulinum is commonly used to selectively inactivate the GTPases RhoA, RhoB, and RhoC, both in vivo and in vitro. C3 Transferase inhibits Rho proteins by ADP-ribosylation on asparagine 41 in the effector binding domain of the GTPase.

TAT-C3 can be used to inactive Rho proteins in fibroblasts, neurons, epithelial, endothelial, and hematopoietic cells as well as other primary and immortalized cell lines.
Research Area Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility
Notes Purification protocol detailed in Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.

References: 7 entries

Rodrigues et al. 2014. Nat Commun. 5:5458. PMID: 25413277.

Croft et al. 2011. Cell Res. 21(4):666-82. PMID: 21079653.

Hanley et al. 2010. Proc Natl Acad Sci U S A. 107(27):12145-50. PMID: 20566876.

Kumar et al. 2009. Mol Cell Biol. 29(15):4262-73. PMID: 19470758.

Busche et al. 2008. J Cell Sci. 121(Pt 7):1025-35. PMID: 18334560.

Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.

Pollock et al. 2005. Cancer Res. 65(4):1244-50. PMID: 15735008.


Add a reference

References: 7 entries

Rodrigues et al. 2014. Nat Commun. 5:5458. PMID: 25413277.

Croft et al. 2011. Cell Res. 21(4):666-82. PMID: 21079653.

Hanley et al. 2010. Proc Natl Acad Sci U S A. 107(27):12145-50. PMID: 20566876.

Kumar et al. 2009. Mol Cell Biol. 29(15):4262-73. PMID: 19470758.

Busche et al. 2008. J Cell Sci. 121(Pt 7):1025-35. PMID: 18334560.

Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.

Pollock et al. 2005. Cancer Res. 65(4):1244-50. PMID: 15735008.


Add a reference

References: 7 entries

Rodrigues et al. 2014. Nat Commun. 5:5458. PMID: 25413277.

Croft et al. 2011. Cell Res. 21(4):666-82. PMID: 21079653.

Hanley et al. 2010. Proc Natl Acad Sci U S A. 107(27):12145-50. PMID: 20566876.

Kumar et al. 2009. Mol Cell Biol. 29(15):4262-73. PMID: 19470758.

Busche et al. 2008. J Cell Sci. 121(Pt 7):1025-35. PMID: 18334560.

Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.

Pollock et al. 2005. Cancer Res. 65(4):1244-50. PMID: 15735008.


Add a reference


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