TAT-C3 (PTD) exoenzyme Vector
Invented by Prof Michael Olson at Cancer Research UK Glasgow: The Beatson Institute
TAT-C3 exoenzyme Vector
Invented by Prof Michael Olson at Cancer Research UK Glasgow: The Beatson Institute
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Info
| Catalogue Number | 152719 |
| Backbone Size (bp) | 5006 |
| Bacterial Resistance | Ampicillin |
| Vector Type | pGEX-KG |
| Antigen/Gene or Protein Targets | C3 ADP-ribosyltransferase |
| Relevance |
TAT-C3 consists of the Clostridium botulinum C3 exozyme (C3 ADP-ribosyltransferase) linked to an HIV TAT leader sequence that permits transduction of the protein across the plasma membrane. The cell penetrating moiety allows rapid and efficient transport through the plasma membrane. Once in the cytosol, the cell penetrating moiety is released, allowing C3 Transferase to freely diffuse intracellularly and inactive RhoA, RhoB, and RhoC, but not related GTPases such as Cdc42 or Rac1. The Exoenzyme C3 Transferase from Clostridium botulinum is commonly used to selectively inactivate the GTPases RhoA, RhoB, and RhoC, both in vivo and in vitro. C3 Transferase inhibits Rho proteins by ADP-ribosylation on asparagine 41 in the effector binding domain of the GTPase. TAT-C3 can be used to inactive Rho proteins in fibroblasts, neurons, epithelial, endothelial, and hematopoietic cells as well as other primary and immortalized cell lines. |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility |
| Notes | Purification protocol detailed in Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655. |
References: 7 entries
Rodrigues et al. 2014. Nat Commun. 5:5458. PMID: 25413277.
Croft et al. 2011. Cell Res. 21(4):666-82. PMID: 21079653.
Hanley et al. 2010. Proc Natl Acad Sci U S A. 107(27):12145-50. PMID: 20566876.
Kumar et al. 2009. Mol Cell Biol. 29(15):4262-73. PMID: 19470758.
Busche et al. 2008. J Cell Sci. 121(Pt 7):1025-35. PMID: 18334560.
Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.
Pollock et al. 2005. Cancer Res. 65(4):1244-50. PMID: 15735008.
Add a reference
References: 7 entries
Rodrigues et al. 2014. Nat Commun. 5:5458. PMID: 25413277.
Croft et al. 2011. Cell Res. 21(4):666-82. PMID: 21079653.
Hanley et al. 2010. Proc Natl Acad Sci U S A. 107(27):12145-50. PMID: 20566876.
Kumar et al. 2009. Mol Cell Biol. 29(15):4262-73. PMID: 19470758.
Busche et al. 2008. J Cell Sci. 121(Pt 7):1025-35. PMID: 18334560.
Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.
Pollock et al. 2005. Cancer Res. 65(4):1244-50. PMID: 15735008.
Add a reference
References: 7 entries
Rodrigues et al. 2014. Nat Commun. 5:5458. PMID: 25413277.
Croft et al. 2011. Cell Res. 21(4):666-82. PMID: 21079653.
Hanley et al. 2010. Proc Natl Acad Sci U S A. 107(27):12145-50. PMID: 20566876.
Kumar et al. 2009. Mol Cell Biol. 29(15):4262-73. PMID: 19470758.
Busche et al. 2008. J Cell Sci. 121(Pt 7):1025-35. PMID: 18334560.
Sahai et al. 2006. Methods Enzymol. 406:128-40. PMID: 16472655.
Pollock et al. 2005. Cancer Res. 65(4):1244-50. PMID: 15735008.
Add a reference
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