Anti-MERS N [9H10]
Invented at A*STAR Accelerate Technologies Pte Ltd
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![Western blotting was performed on full length N proteins (with a N-terminal myc-tag) of different human coronaviruses prepared from overexpressing 293FT cells (Lane 1: Untransfected, Lane 2: 229E-N, Lane 3: HKU1-N, Lane 4:NL63-N, Lane 5: OC43-N, Lane 6: MERS-N, Lane 7: MERS-N, Lane 8: SARS-N) using anti-MERS N [9H10] or an anti-Myc antibody (lower panel). Anti-MERS N [9H10] bound specifically to the N protein from MERS-CoV (lanes 6 and 7) but not to N proteins of other coronavirus.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/3cbf2d0e-df4d-424b-ba2a-9e8c93574429.png)
![Immunofluorecence was performed on cos7 cells mock-transfected (left panel), transfected with plasmid containing either the SARS N (middle panel) or MERS N (right panel) open reading frame. At 24-48h post transfection, cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X- 100 and stained using Anti-MERS N [9H10] followed by a FITC-conjugated secondary goat anti-mouse (1:100). Nucleic acid staining was done using DAPI (1μg/ml).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/4734a78f-2e0d-4d22-bf8e-4a3a610580dd.png)
![Western blotting was performed on full length N proteins (with a N-terminal myc-tag) of different human coronaviruses prepared from overexpressing 293FT cells (Lane 1: Untransfected, Lane 2: 229E-N, Lane 3: HKU1-N, Lane 4:NL63-N, Lane 5: OC43-N, Lane 6: MERS-N, Lane 7: MERS-N, Lane 8: SARS-N) using anti-MERS N [9H10] or an anti-Myc antibody (lower panel). Anti-MERS N [9H10] bound specifically to the N protein from MERS-CoV (lanes 6 and 7) but not to N proteins of other coronavirus.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/3cbf2d0e-df4d-424b-ba2a-9e8c93574429.png)
![Immunofluorecence was performed on cos7 cells mock-transfected (left panel), transfected with plasmid containing either the SARS N (middle panel) or MERS N (right panel) open reading frame. At 24-48h post transfection, cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X- 100 and stained using Anti-MERS N [9H10] followed by a FITC-conjugated secondary goat anti-mouse (1:100). Nucleic acid staining was done using DAPI (1μg/ml).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/4734a78f-2e0d-4d22-bf8e-4a3a610580dd.png)
![Image thumbnail for Anti-MERS N [9H10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/3cbf2d0e-df4d-424b-ba2a-9e8c93574429.png)
![Image thumbnail for Anti-MERS N [9H10]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/4734a78f-2e0d-4d22-bf8e-4a3a610580dd.png)
| Catalogue Number | 152663 |
| Applications | IF WB |
| Antigen/Gene or Protein Targets | MERS coronaviurs nucleocapsid protein |
| Reactivity | Virus |
| Relevance | A novel coronavirus (termed as severe acute respiratory syndrome coronavirus, SARS-CoV) was the cause of a viral outbreak which caused profound disturbances worldwide in 2003. Most recently, another novel coronavirus has achieved successful zoonotic transmission and been named as the Middle East respiratory syndrome coronavirus (MERS-CoV). As of 9 May 2014, a total of 536 people have been infected and the fatality rate is ~27%. As the Nucleocapsid protein (N) protein of coronavirus is an abundant virion protein and highly immunogenic, monoclonal antibody for MERS N will be useful for the detection of the virus especially if the monoclonal antibody can distinguish MERS-CoV from other human coronaviruses. |
| Host | Mouse |
| Immunogen | GST-MERS N fusion |
| Subclass | Inconclusive |
| Subclass Notes | Negative results for most recent batch |
| Myeloma Used | Sp2/0-Ag14 |
| Research Area | Virology |
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