Anti-Cytochrome P450 27A1 [V29-P4B8*B2]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (2)
- Inventor Info
Info
![Western blot was performed using anti-Cytochrome P450 27A1 [V29-P4B8*B2]. The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well.
No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/9d87175d-faa7-48d9-beea-28863df0c679.png)
![Western blot was performed using anti-Cytochrome P450 27A1 [V29-P4B8*B2]. The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well.
No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/9d87175d-faa7-48d9-beea-28863df0c679.png)
![Image thumbnail for Anti-Cytochrome P450 27A1 [V29-P4B8*B2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/16d2818c-12da-4fe4-94fe-cc92fd8cd7e0.png)
![Image thumbnail for Anti-Cytochrome P450 27A1 [V29-P4B8*B2]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/9d87175d-faa7-48d9-beea-28863df0c679.png)
| Catalogue Number | 152599 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Cytochrome P450, Family 27, Subfamily A, Polypeptide 1 (CYP27A1), Sterol 27-Hydroxylase |
| Reactivity | Human |
| Relevance | Cytochrome P450 27A1 (CYP27A1) catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide KVVLAPETGEL (C-terminal sequence) |
| Positive Control | IHC: formalin-fixed, paraffin-embedded human sections; WB: Overexpression lysates |
| Subclass | IgG1 kappa |
| Notes |
For IHC perform antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0 This antibody was applied in a poster presented at the 2015 NCRI conference: http://abstracts.ncri.org.uk/?s=oxysterol&year_published=2015 |
| Research Area | Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism |
References: 2 entries
Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer.
Europe PMC ID: 27341022
Swan et al. 2016. Oncotarget. :. PMID: 27341022.
IHC WB
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References: 2 entries
Characterisation of the oxysterol metabolising enzyme pathway in mismatch repair proficient and deficient colorectal cancer.
Swan et al. 2016. Oncotarget. :. PMID: 27341022.
IHC WB
Add a reference
