Anti-CD49b [16B4]
Invented at University of Manchester
- Datasheet
- References (10)
- Inventor Info
Info
| Catalogue Number | 152483 |
| Applications | ELISA FACS IHC IF IP |
| Antigen/Gene or Protein Targets | CD49f (Integrin a2+b1) |
| Synonyms | Integrin Subunit Alpha 2; Alpha 2 Subunit Of VLA-2 Receptor; Platelet Membrane Glycoprotein Ia; CD49 Antigen-Like Family Member B; Collagen Receptor; CD49B; GPIa; Very Late Activation Protein 2 Receptor; Alpha-2 Subunit; Human Platelet Alloantigen System; Platelet Glycoprotein GPIa; VLA-2 Subunit Alpha; CD49b Antigen; HPA-5; VLA-2; VLAA2; BR |
| Reactivity | Human |
| Relevance | CD49b is an integrin alpha subunit which makes up half of the α2β1 integrin duplex. Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. They are found on a wide variety of cell types including, T cells (the NKT cells), NK cells, fibroblasts and platelets. Integrins are involved in cell adhesion and also participate in cell-surface mediated signalling. |
| Host | Mouse |
| Immunogen | Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract. |
| Positive Control | In IF, this antibody gave a positive signal in HepG2 cells. |
| Subclass | IgG1 |
| Myeloma Used | P3X63Ag8.653 |
| Notes | 16B4 can be used to immunoprecipitate a non-reducible alpha subunit from I(125) surface labelled Human cell line extract. It is specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots. |
| Research Area | Adhesion, Cell Signaling & Signal Transduction, Immunology |
References: 10 entries
Suarez et al. 2015. Arch Dermatol Res. 307(2):115-33. PMID: 25322916.
Menhofer et al. 2014. Cardiovasc Res. 104(2):303-14. PMID: 25239826.
Identification of biomarkers involved in differential profiling of hypertrophic and keloid scars versus normal skin.
Europe PMC ID: 25322916
In vitro and in vivo characterization of the actin polymerizing compound chondramide as an angiogenic inhibitor.
Europe PMC ID: 25239826
Tuckwell et al. 2000. Biochem J. 350 Pt 2:485-93. PMID: 10947963.
Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA domain as a functionally important region within alpha2beta1.
Europe PMC ID: 10947963
Sincock et al. 1999. J Cell Sci. 112 ( Pt 6):833-44. PMID: 10036233.
PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.
Europe PMC ID: 10036233
Fitter et al. 1999. Biochem J. 338 ( Pt 1):61-70. PMID: 9931299.
Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.
Europe PMC ID: 9931299
Add a reference
References: 10 entries
Suarez et al. 2015. Arch Dermatol Res. 307(2):115-33. PMID: 25322916.
Menhofer et al. 2014. Cardiovasc Res. 104(2):303-14. PMID: 25239826.
Identification of biomarkers involved in differential profiling of hypertrophic and keloid scars versus normal skin.
In vitro and in vivo characterization of the actin polymerizing compound chondramide as an angiogenic inhibitor.
Tuckwell et al. 2000. Biochem J. 350 Pt 2:485-93. PMID: 10947963.
Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA domain as a functionally important region within alpha2beta1.
Sincock et al. 1999. J Cell Sci. 112 ( Pt 6):833-44. PMID: 10036233.
PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.
Fitter et al. 1999. Biochem J. 338 ( Pt 1):61-70. PMID: 9931299.
Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.
Add a reference
