HEK293-A mRFP-ATG9 Cell Line
Invented by Dr Sharon Tooze from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151828 |
| Antigen/Gene or Protein Targets | ATG9A |
| Parental Line | HEK293-A |
| Host | Human |
| Tissue | Embryo |
| Model | Reporter |
| Relevance | The HEK293-A mRFP-ATG9 cell line is a stable cell line expressing mRFP-tagged ATG9 (human) in background of HEK293A cell line. It can be used to monitor delivery of membrane from the endosomes and Golgi to the forming autophagosome using live-cell microscopy and biochemical approaches. |
| Production Details | pEGFP-mRFP-Atg9 plasmid was transfected into HEK293A cells. The plasmid was generated by excision of the EGFP coding sequence from pEGFP-C1 and insertion of the mRFP-ATG9 cassette. The cells were selected with 800ug/ml geneticin (G418) followed by single cell clonal dilution. Clones were screened by confocal microscopy for low-level expression in which mRFP- Atg9 distribution resembled that of endogenous mAtg9 and by western blot. Clone 1F10 was selected as the best based on these parameters. |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Cell Signaling & Signal Transduction, Cellular Transport, Drug Discovery & Development, Metabolism |
| Recommended Growing Conditions | DMEM + 10% FCS + 2mM Glutamine + 400ug/ml geneticin (G418) for maintainance. |
| Notes | HEK293 cells were obtained under licence from AdVec |
| Cellosaurus ID | CVCL_W349 |
References: 4 entries
Orsi et al. 2012. Mol Biol Cell. 23(10):1860-73. PMID: 22456507.
Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy.
Europe PMC ID: 22456507
Young et al. 2006. J Cell Sci. 119(Pt 18):3888-900. PMID: 16940348.
Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes.
Europe PMC ID: 16940348
Add a reference
References: 4 entries
Orsi et al. 2012. Mol Biol Cell. 23(10):1860-73. PMID: 22456507.
Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy.
Young et al. 2006. J Cell Sci. 119(Pt 18):3888-900. PMID: 16940348.
Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes.
Add a reference
