PEO1 Cell Line
Invented by Simon Langdon at Cancer Research UK Edinburgh Centre
PEO1-CDDP Cell Line
Invented by Simon Langdon from The University of Edinburgh
Invented at Cancer Research UK Edinburgh Centre
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151784 |
| Antigen/Gene or Protein Targets | Human ovarian cancer; oestrogen receptor, drug resistance, cisplatin resistance |
| Parental Line | PEO1 |
| Host | Human |
| Tissue | Ovary |
| Disease Keywords | Adenocarcinoma |
| Model | Tumour line |
| Relevance | The PEO1-CDDP Cell Line is an in-vitro model to understand the changes in EGF signalling after the onset of cisplatin resistance. Coupled with this change in drug sensitivity, this model also has a modified responsiveness to ligands of the erbB receptor family. This model will be helpful in studying the changes in cellular signalling after drug treatment, which are currently attracting a great deal of interest and this model will be of particular relevance when cytotoxic therapy is combined with signalling inhibitors. |
| Production Details | The PE01CDDP cell line was established by in vitro exposure of the PE01 line to cisplatin commencing at 25 nmol/L cisplatin and increasing to 1 Amol/L over a period of 4 months. The resistant cell line thus obtained was then passaged in the absence of cisplatin for a further 4 months to ensure that the resistance remained stable. PEO1 CDDP is from the same patient as the PEO1, PEO4 and PEO6 cell lines (ECACC Catalogue numbers 10032308, 10032309 & 10032310). |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Drug Discovery & Development |
| Recommended Growing Conditions | RPMI 1640 containing 10% heat-inactivated FCS, penicillin (100 units/mL), and streptomycin (100 ug/mL). Cells were maintained routinely at 37oC in a humidified atmosphere of 5% CO2 in air. |
| Notes | Cisplatin Resistance |
| Cellosaurus ID | CVCL_Y032 |
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Invented by Simon Langdon at Cancer Research UK Edinburgh Centre
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Invented by Simon Langdon at Cancer Research UK Edinburgh Centre
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PEO23 Cell Line
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Invented by Simon Langdon at Cancer Research UK Edinburgh Centre
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References: 2 entries
Macleod et al. 2005. Cancer Res. 65(15):6789-800. PMID: 16061661.
Altered ErbB receptor signaling and gene expression in cisplatin-resistant ovarian cancer.
Europe PMC ID: 16061661
Add a reference
References: 2 entries
Macleod et al. 2005. Cancer Res. 65(15):6789-800. PMID: 16061661.
Altered ErbB receptor signaling and gene expression in cisplatin-resistant ovarian cancer.
Add a reference
