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C99 Colorectal adenocarcinoma Cell Line

Invented by Prof Walter Bodmer from University of Oxford
Invented at University of Oxford

Info

Catalogue Number 151761
Host Human
Tissue Colon
Disease Keywords Adenocarcinoma
Model Tumour line
Relevance The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes' stage C
Research Area Cancer, Drug Discovery & Development
Recommended Growing Conditions Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. culture medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
Cellosaurus ID CVCL_8176

References

There are 2 reference entries for this reagent.

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References: 2 entries

Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639.

Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells.

Europe PMC ID: 10051639


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References: 2 entries

Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639.

Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells.


Add a reference