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C84 Colorectal adenocarcinoma Cell Line

Invented by Prof Walter Bodmer from University of Oxford
Invented at University of Oxford

Info

Catalogue Number 151760
Host Human
Tissue Colon
Model Tumour line
Relevance The C84 cell line was established from a 67-year old male patient with a poorly differentiated adenocarcinoma of the caecum classified as Dukes' stage C.
Research Area Cancer, Drug Discovery & Development
Recommended Growing Conditions Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
Cellosaurus ID CVCL_5250

References

There are 2 reference entries for this reagent.

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References: 2 entries

Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.

Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.

Europe PMC ID: 8464898


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References: 2 entries

Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.

Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.


Add a reference