PKCepsilon Mouse
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151662 |
| Antigen/Gene or Protein Targets | PKC epsilon |
| Relevance | Investigating the biological role of PKCepsilon (e.g. required for macrophage activation and defence against bacterial infection, roles in cell division). Mouse line with a homozygous disruption of the PKCepsilon locus. PKCe-/- animals appear normal and are generally healthy, though the female mice frequently develop a bacterial infection of the uterus. Macrophages from the PKCe-/- mice demonstrate a severely attenuated response to LPS and IFNg. Mice have a significantly decreased survival when challenged iv with Gram -ve or +ve bacteria. |
| Production Details | A 9.3-kb EcoRI fragment containing the first exon of the PKCe gene was isolated from a murine 129/Sv genomic DNA library. A targeting vector was generated by introducing a positive selectable cassette into the PstI site of exon 1. This cassette contains stop codons in all three frames, an independent ribosomal entry site (IRES) followed by the LacZ gene with an SV40 polyadenylation sequence, and a neomycin phosphotransferase gene (MC1Neo poly(A). Correctly targeted GK129 ES cells were injected into C57BL/6 blastocysts. Chimeric mice were bred to C57BL/6 mice in specific pathogen-free (SPF) conditions to generate PKCe homozygous mutant animals. PKCe-/- and PKCe+/- mice were maintained on a mixed C57Bl/6 129/Sv genetic background and were genotyped by Southern blot or PCR analysis of tail DNA. Mice were fed ad libitum with a standard diet (Panlab) and kept under a light and dark cycle of 12 h (lights on at 8 a.m.). |
| Conditional | No |
| Mouse Genetic Background/Cross History | C57BL/6 x 129/Sv |
| Zygosity | Homozygous |
| Research Area | Genetic Studies Tools, Immunology |
References: 2 entries
Castrillo et al. 2001. J Exp Med. 194(9):1231-42. PMID: 11696589.
Protein kinase Cepsilon is required for macrophage activation and defense against bacterial infection.
Europe PMC ID: 11696589
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References: 2 entries
Castrillo et al. 2001. J Exp Med. 194(9):1231-42. PMID: 11696589.
Protein kinase Cepsilon is required for macrophage activation and defense against bacterial infection.
Add a reference
