Anti-WIPI2 [2A2]
Invented by Dr Sharon Tooze from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (10)
- Inventor Info
Info
| Catalogue Number | 151651 |
| Applications | FACS IF IP WB |
| Antigen/Gene or Protein Targets | WIPI2 |
| Reactivity | Human and Mouse |
| Relevance | This antibody is useful for the detection of WIPI-2, an essential protein for autophagy. WIPI2 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. WIPI2 is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. WIPI-2 is the mammalian homologue of Atg18. |
| Host | Mouse |
| Immunogen | Synthetic peptide corresponding to C-terminus of WIPI2b CSALRLDEDSEHPPMILRTD |
| Positive Control | HEK293 cells |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 49 |
| Recommended Growing Conditions | DMEM + 15% FCS |
| Notes | Autophagosomes only detectable after induction of autophagy. |
| Research Area | Cancer, Cardiovascular, Protein Degradation |
References: 10 entries
Nishimura et al. 2017. EMBO J. 36(12):1719-1735. PMID: 28495679.
IF
Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains.
Europe PMC ID: 28495679
Martinez-Martin et al. 2017. Science. 355(6325):641-647. PMID: 28183981.
FACS
A switch from canonical to noncanonical autophagy shapes B cell responses.
Europe PMC ID: 28183981
Polson et al. 2010. Autophagy. 6(4):506-22. PMID: 20505359.
Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
Europe PMC ID: 20505359
Li et al. 2005. J Biol Chem. 280(29):26941-52. PMID: 15917248.
Krüppel-like factor-6 promotes preadipocyte differentiation through histone deacetylase 3-dependent repression of DLK1.
Europe PMC ID: 15917248
Benzeno et al. 2004. Cancer Res. 64(11):3885-91. PMID: 15172998.
Cyclin-dependent kinase inhibition by the KLF6 tumor suppressor protein through interaction with cyclin D1.
Europe PMC ID: 15172998
Add a reference
References: 10 entries
Nishimura et al. 2017. EMBO J. 36(12):1719-1735. PMID: 28495679.
IF
Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains.
Martinez-Martin et al. 2017. Science. 355(6325):641-647. PMID: 28183981.
FACS
A switch from canonical to noncanonical autophagy shapes B cell responses.
Polson et al. 2010. Autophagy. 6(4):506-22. PMID: 20505359.
Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
Li et al. 2005. J Biol Chem. 280(29):26941-52. PMID: 15917248.
Krüppel-like factor-6 promotes preadipocyte differentiation through histone deacetylase 3-dependent repression of DLK1.
Benzeno et al. 2004. Cancer Res. 64(11):3885-91. PMID: 15172998.
Cyclin-dependent kinase inhibition by the KLF6 tumor suppressor protein through interaction with cyclin D1.
Add a reference
