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Monoclonal Antibodies

Anti-Metapneumovirus Fusion Protein [hMPV24]

Invented at University of Newcastle upon Tyne
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Image thumbnail for Anti-Metapneumovirus Fusion Protein [hMPV24]
Image thumbnail for Anti-Metapneumovirus Fusion Protein [hMPV24]
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Adapted from El Najjar et al. 2016. PLoS Pathog; 12(9):e1005922. PMID: 27683250. Figure. HMPV infected BEAS-2B cells at 18 or 24 h.p.i., were fixed and processed for immunofluorescence stained with antibody against for HMPV N protein, P protein and F protein (hMPV24).
Adapted from Cifuentes-Muñoz et al. 2017; J Virol. 91(24). PMID: 28978704. Figure. BEAS-2B cells were infected with HMPV CAN97-83 at an MOI of 3 PFU/cell and fixed at 24 hpi. FISH and immunofluorescence were performed to detect vRNA (red), +RNA (purple; green as a pseudocolor), polyadenylated RNAs (red), and the HMPV F proteins (green). Bars, 10 μm.
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Catalogue Number 151640
Applications IHC IF WB
Antigen/Gene or Protein Targets Metapneumovirus Fusion Protein
Reactivity Virus
Relevance Monoclonal antibody which can identify human metapneumovirus via immunofluorescent staining and does not cross react to other cell cultures

Background and Research Application
Anti-hMPV monoclonal antibody which can identify and locate the fusion glycoprotein of human metapneumovirus (hMPV) of both types A and B, within a sample.
hMPV is a negative single stranded RNA virus of the paramyxoviridae family and can cause upper and lower respiratory tract infections. Discovered in 2001, there appear to be 4 lineages of the hMPV virus, stemming from sub-groups A and B.Anti-hMPV24 can recognise the fusion protein of all 4 lineages(A1,A2, B1 and B2). Anti-hMPV24 be also used in conjunction with other hMPV antibody clones to detect all strains of the virus.
Anti-hMPV24 was created to diagnose hMPV infection in respiratory specimens via immunofluorescence staining with optimal staining of clinical samples with low backgrounds on uninfected specimens.
Host Mouse
Immunogen Human MPV strain NCL-145
Positive Control Human MPV infected LLC-MK2 cells
Subclass IgG2a
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + Hepes (25mM) + glutamine (2mM) + 10% heat inactivated FCS.
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Recommended concentration for immunofluorescence: 20ug/ml

Reactivity: Metapneumovirus (MPV)
Anti-hMPV24 does not react with cell cultures infected with hRSV, influenzavirus A or B, adenovirus, parainfluenza viruses 1-4, mumps virus, measles virus, varicella-zoster virus, herpes simplex virus types 1 and 2, human cytomegalovirus, human herpes virus type 6, ECHOvirus 19, Coxsachievirus B4, poliovirus types1–3. HHV6 and against uninfected HeLa, MA104, 3MK, LLC-MK, HEp2, MRC-5 and HSB-2 cells

Concentration
2mg/ml
Research Area Virology

References

There are 6 reference entries for this reagent.

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References: 6 entries

Cifuentes-Muñoz et al. 2017. J Virol. 91(24):. PMID: 28978704.

IF

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El Najjar et al. 2016. PLoS Pathog. 12(9):e1005922. PMID: 27683250.

IF

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Tedcastle et al. 2012. J Med Virol. 84(7):1061-70. PMID: 22585723.

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The characterization of monoclonal antibodies to human metapneumovirus and the detection of multiple forms of the virus nucleoprotein and phosphoprotein.

Europe PMC ID: 22585723

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Fenwick et al. 2007. J Clin Virol. 40(3):193-6. PMID: 17869169.

WB

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Diagnosis of human metapneumovirus by immunofluorescence staining with monoclonal antibodies in the North-East of England.

Europe PMC ID: 17869169

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Add a reference

References: 6 entries

Cifuentes-Muñoz et al. 2017. J Virol. 91(24):. PMID: 28978704.

IF

View  

El Najjar et al. 2016. PLoS Pathog. 12(9):e1005922. PMID: 27683250.

IF

View  

Tedcastle et al. 2012. J Med Virol. 84(7):1061-70. PMID: 22585723.

View  

The characterization of monoclonal antibodies to human metapneumovirus and the detection of multiple forms of the virus nucleoprotein and phosphoprotein.

View  

Fenwick et al. 2007. J Clin Virol. 40(3):193-6. PMID: 17869169.

WB

View  

Diagnosis of human metapneumovirus by immunofluorescence staining with monoclonal antibodies in the North-East of England.

View  

Add a reference

Inventor Information

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Invented at

University of Newcastle upon Tyne
University of Newcastle upon Tyne

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