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Monoclonal Antibodies

Anti-RSV G Glycoprotein [133]

Invented at University of Newcastle upon Tyne
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Image thumbnail for Anti-RSV G Glycoprotein [133]
Image thumbnail for Anti-RSV G Glycoprotein [133]
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Adapted from Robinson et al. 2014. J Med Virol. 86(7):1267-77. PMID: 24415460. Figure. Reduced SDS–PAGE Western blot of untreated (8/60) and tunicamycin treated hRSV8/60 (8/60-Tu) stained with MAb 133 and MAb 21. A lane loaded with uninfected HeLa cell lysate (HeLa) was run as a negative control in each electrophoretogram.
Adapted from Shi et al. 2016. Sci Rep. 6:35851. PMID: 27767097. Figure. Baicalin treatment suppresses RSV F and G protein expression. HEp-2 cells were infected with RSV at 1 MOI in the presence or absence or baicalin at indicated concentrations for 36 hr. RSV-G and F protein expression was detected with specific antibodies. GAPDH was used as a loading control.
Adapted from Vanover et al. 2017. Nat Commun. 8(1):667. PMID: 28939853. Figure. Vero cells transfected for 24 h with RSV G (top) and RSV F (bottom) mRNA were labeled with SBA-488 at 4 °C (green) before being fixed and stained without permeabilization for RSV G or RSV F (red) and nuclei (gray).
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Catalogue Number 151639
Applications ELISA IHC IF WB
Antigen/Gene or Protein Targets Respiratory syncytial virus subgroup A G glycoprotein
Reactivity Virus
Relevance Identification and location of expression of the G glycoprotein of HRSV of both sub-groups A and B . This antibody confers passive protection against HRSV of both subgroups in an animal model of hRSV infection.
Host Mouse
Immunogen Human RSV strain A2 infected HeLa cells
Positive Control Human RSV A2 infected HeLa cells
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + 10% FCS + Hepes (25mM) + glutamine (2mM)
Notes Reactivity: Respiratory Syncytial Virus
Research Area Virology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Vanover et al. 2017. Nat Commun. 8(1):667. PMID: 28939853.

IF

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Shi et al. 2016. Sci Rep. 6:35851. PMID: 27767097.

WB

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Robinson et al. 2014. J Med Virol. 86(7):1267-77. PMID: 24415460.

WB

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Generation and epitope mapping of a sub-group cross-reactive anti-respiratory syncytial virus G glycoprotein monoclonal antibody which is protective in vivo.

Europe PMC ID: 24415460

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References: 4 entries

Vanover et al. 2017. Nat Commun. 8(1):667. PMID: 28939853.

IF

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Shi et al. 2016. Sci Rep. 6:35851. PMID: 27767097.

WB

View  

Robinson et al. 2014. J Med Virol. 86(7):1267-77. PMID: 24415460.

WB

View  

Generation and epitope mapping of a sub-group cross-reactive anti-respiratory syncytial virus G glycoprotein monoclonal antibody which is protective in vivo.

View  

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Inventor Information

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Invented at

University of Newcastle upon Tyne
University of Newcastle upon Tyne

39 reagents listed on Ximbio