C-Raf KI D486A Cell Line
Invented at University Of Leicester
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151631 |
| Antigen/Gene or Protein Targets | Raf1 D486A oncogenic mutant |
| Host | Mouse |
| Tissue | Embryo |
| Disease Keywords | Cancer |
| Model | Knock-In |
| Relevance | The C-Raf KI D486A Cell Line is derived from the knock-in mouse model carrying oncogenic Raf-1 D486A; it is useful for in vivo study of oncogenic Raf-1 mutants and Ras signalling. |
| Production Details | Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture. |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Drug Discovery & Development |
| Recommended Growing Conditions | DMEM, 10% FCS, 100U/mol penicillin/streptomycin |
| Notes | See the Raf-1 D486A oncogenic mutant knockin mouse that these cells are produced from. |
References: 2 entries
Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.
CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.
Europe PMC ID: 18922468
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References: 2 entries
Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.
CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.
Add a reference
