Anti-Talin1 [93E12]
Invented at University Of Leicester
Anti-Talin1 [97H6]
Invented at University Of Leicester
- Datasheet
- References (12)
- Inventor Info
Info
| Catalogue Number | 151627 |
| Applications | ELISA IHC IF IP WB |
| Antigen/Gene or Protein Targets | Talin 1 |
| Reactivity | Human |
| Relevance |
Monoclonal antibody for investigation into cell adhesion with no cross reactivity with related talin 2 protein. Background and Research Application Talin 1 is a high molecular weight protein found in a variety of tissues and cell types. Talin 1 is essential for integrin-mediated cell adhesion, the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. Talin 1 also links lymphocytes at cell-cell contact points. The N-terminus of talin 1 contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. This antibody recognizes an epitope within the region of amino acids 482-655 of human talin-1. Anti-talin1 antibody can be used for specific detection of the focal adhesion protein talin 1. |
| Host | Mouse |
| Immunogen | His-tagged polypeptide containing residues 489-911 of mouse Talin1 |
| Immunogen UniProt ID | Q9Y490 |
| Positive Control | Fibroblasts |
| Subclass | IgG1 |
| Myeloma Used | NS0 |
| Recommended Growing Conditions | RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2 |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Suggested dilutions: 1/100 - 1/1000 Anti-Talin1 antibody does not cross react with talin 2. Concentration 1mg/ml as standard |
| Research Area | Adhesion, Cell Signaling & Signal Transduction |
References: 12 entries
Li et al. 2017. Oncotarget. 8(63):106876-106887. PMID: 29290996.
Bouchet et al. 2016. Elife. 5:. PMID: 27410476.
Debrand et al. 2012. Biochem Biophys Res Commun. 426(2):190-5. PMID: 22925892.
IHC WB
Mice carrying a complete deletion of the talin2 coding sequence are viable and fertile.
Europe PMC ID: 22925892
Praekelt et al. 2012. Eur J Cell Biol. 91(3):180-91. PMID: 22306379.
New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.
Europe PMC ID: 22306379
Liu et al. 2011. Mol Cell Biol. 31(16):3366-77. PMID: 21670148.
WB
Talin1 regulates integrin turnover to promote embryonic epithelial morphogenesis.
Europe PMC ID: 21670148
Monkley et al. 2011. Dev Biol. 349(2):494-502. PMID: 21081121.
IHC WB
Endothelial cell talin1 is essential for embryonic angiogenesis.
Europe PMC ID: 21081121
Kopp et al. 2010. Eur J Cell Biol. 89(9):661-73. PMID: 20605055.
IF
Studies on the morphology and spreading of human endothelial cells define key inter- and intramolecular interactions for talin1.
Europe PMC ID: 20605055
Add a reference
References: 12 entries
Li et al. 2017. Oncotarget. 8(63):106876-106887. PMID: 29290996.
Bouchet et al. 2016. Elife. 5:. PMID: 27410476.
Debrand et al. 2012. Biochem Biophys Res Commun. 426(2):190-5. PMID: 22925892.
IHC WB
Mice carrying a complete deletion of the talin2 coding sequence are viable and fertile.
Praekelt et al. 2012. Eur J Cell Biol. 91(3):180-91. PMID: 22306379.
New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2.
Liu et al. 2011. Mol Cell Biol. 31(16):3366-77. PMID: 21670148.
WB
Talin1 regulates integrin turnover to promote embryonic epithelial morphogenesis.
Monkley et al. 2011. Dev Biol. 349(2):494-502. PMID: 21081121.
IHC WB
Endothelial cell talin1 is essential for embryonic angiogenesis.
Kopp et al. 2010. Eur J Cell Biol. 89(9):661-73. PMID: 20605055.
IF
Studies on the morphology and spreading of human endothelial cells define key inter- and intramolecular interactions for talin1.
Add a reference
