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DX3 beta6-puro Cell Line


Catalogue Number 151625
Antigen/Gene or Protein Targets Beta 6 integrin
Parental Line DX3
Host Human
Disease Keywords Melanoma
Model Knock-Out
Relevance DX3 puro-beta6 Cell Line is a melanoma cell line endogenously expressing a range of integrins, but not beta 6. The use of DX3-puro/beta6 as a matched pair with the DX3-puro cell line is a useful tool for studying beta 6 function or specificity of agents that interfere with beta 6.
Production Details As described in Hausner et al. Cancer Research (2007) Vol 67, p7833-7840. The human melanoma cell line DX3 was infected with pBabe retroviruses encoding puromycin resistance or, in addition, cDNA for human integrin beta 6. Cells were selected in puromycin (1.25 ug/mL) followed by magnetic bead sorting, using 10D5 (mouse anti-avh6; Chemicon International), according to the manufacturer’s instructions (Dynal; Invitrogen). When growing these lines the antibiotic does not have to be there all the time. The avb6 expression can drift so recipients must check by flow cytometery after they have been in continuous passage, at least once a month. Use the above magnetic beads to re-select when this happens.
Research Area Adhesion, Cancer, Cell Signaling & Signal Transduction, Drug Discovery & Development
Growth/Phenotype Keywords DX3 melanoma cell line stably transfected with the integrin beta 6
Recommended Growing Conditions DMEM + 10%FCS/1.25ug/ml puromycin. Antibiotic is not necessary all the time.
Notes Useful model for studying the function and activity of beta 6 integrin when used as a matched pair with the parent DX3 line. DX3 cells endogenously express a variety of integrins, but not beta 6. These cell lines have been used to validate the activity and selectivity of AvB6 binding peptides.
Cellosaurus ID CVCL_1D33

References: 3 entries

Hausner et al. 2009. Cancer Res. 69(14):5843-50. PMID: 19549907.

Hausner et al. 2009. Mol Imaging. 8(2):111-21. PMID: 19397856.

Hausner et al. 2007. Cancer Res. 67(16):7833-40. PMID: 17699789.

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