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- References (10)
- Inventor Info
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![Western blotting on an array of lysates prepared from cultured cells using anti-RIP140 [6D7]. Antibody dilution: 1/500, incubated o/n in PBS+NaN3. Secondary: anti-mouse HRP, 1/2000, 1h. Exposure: 1min](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/f1234c16-ce81-4f83-97be-1233395de777.jpg)
![Western blotting on an array of lysates prepared from cultured cells using anti-RIP140 [6D7]. Antibody dilution: 1/500, incubated o/n in PBS+NaN3. Secondary: anti-mouse HRP, 1/2000, 1h. Exposure: 1min](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/f1234c16-ce81-4f83-97be-1233395de777.jpg)
![Image thumbnail for Anti-RIP140 [6D7]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/f1234c16-ce81-4f83-97be-1233395de777.jpg)
| Catalogue Number | 151614 |
| Applications | ChIP ELISA IF IP WB |
| Antigen/Gene or Protein Targets | RIP140 |
| Reactivity | Human, Mouse and Rat |
| Relevance | RIP140 is a ligand-dependent corepressor for most nuclear receptors, and functions through interaction with their AF2 activation domains. Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. |
| Host | Mouse |
| Immunogen | Recombinant human RIP140 GST fusion protein |
| Positive Control | Hela cells |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | RPMI + 5% FCS |
| Strain | Balb/c |
| Notes | Recommmended antibody incubation for western blotting: 1/500 |
| Research Area | Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling |
References: 10 entries
Foulds et al. 2013. Mol Cell. 51(2):185-99. PMID: 23850489.
WB
Proteomic analysis of coregulators bound to ERα on DNA and nucleosomes reveals coregulator dynamics.
Europe PMC ID: 23850489
Poliandri et al. 2011. J Biol Rhythms. 26(3):187-99. PMID: 21628546.
ChIP
Modulation of clock gene expression by the transcriptional coregulator receptor interacting protein 140 (RIP140).
Europe PMC ID: 21628546
Hallberg et al. 2008. Mol Cell Biol. 28(22):6785-95. PMID: 18794372.
WB
A functional interaction between RIP140 and PGC-1alpha regulates the expression of the lipid droplet protein CIDEA.
Europe PMC ID: 18794372
Herzog et al. 2007. Mol Endocrinol. 21(11):2687-97. PMID: 17684114.
Kiskinis et al. 2007. EMBO J. 26(23):4831-40. PMID: 17972916.
WB
RIP140 directs histone and DNA methylation to silence Ucp1 expression in white adipocytes.
Europe PMC ID: 17972916
The nuclear receptor cofactor, receptor-interacting protein 140, is required for the regulation of hepatic lipid and glucose metabolism by liver X receptor.
Europe PMC ID: 17684114
Add a reference
References: 10 entries
Foulds et al. 2013. Mol Cell. 51(2):185-99. PMID: 23850489.
WB
Proteomic analysis of coregulators bound to ERα on DNA and nucleosomes reveals coregulator dynamics.
Poliandri et al. 2011. J Biol Rhythms. 26(3):187-99. PMID: 21628546.
ChIP
Modulation of clock gene expression by the transcriptional coregulator receptor interacting protein 140 (RIP140).
Hallberg et al. 2008. Mol Cell Biol. 28(22):6785-95. PMID: 18794372.
WB
A functional interaction between RIP140 and PGC-1alpha regulates the expression of the lipid droplet protein CIDEA.
Herzog et al. 2007. Mol Endocrinol. 21(11):2687-97. PMID: 17684114.
Kiskinis et al. 2007. EMBO J. 26(23):4831-40. PMID: 17972916.
WB
RIP140 directs histone and DNA methylation to silence Ucp1 expression in white adipocytes.
The nuclear receptor cofactor, receptor-interacting protein 140, is required for the regulation of hepatic lipid and glucose metabolism by liver X receptor.
Add a reference
Inventor Information
Inventors
|
Bill Gullick |
