Anti-ENTPD1 [AC2]
Invented by Prof Martin Rowe from University Of Birmingham
Invented at University Of Birmingham
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151580 |
| Applications | FACS IF IP WB |
| Antigen/Gene or Protein Targets | Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1, CD39) |
| Reactivity | Human |
| Relevance |
Monoclonal antibody which binds ENTPD1, and reacts with Epstein Barr lymphoblastoid cell lines. Background and Research Application ENTPD1 is a transmembrane glycoprotein of apparent molecular weight of 70-100 kD that has an Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1, ecto-apyrase) activity hydrolysing the alpha- and beta- phosphate residues of extracellular ATP or ADP. In haematopoietic tissues, it is primarily expressed on activated B, T and NK cells. It is also expressed on some endothelial cells and cardiac neurons, and helps regulate platelet activation, vascular inflammation, and cardiac norepinephrine release. ACE2 reacted consistently with all Epstein Barr virus lymphoblastoid cell line (EB‐LCL) tested, with a subpopulation of cells in some but not all EB virus genome‐positive Burkitt lymphoma lines, but with none of a range of EB virus genome‐negative cell lines of lymphoma or leukaemia origin. |
| Host | Mouse |
| Immunogen | EBV-transformed human B lymphoblastoid cell line |
| Immunogen UniProt ID | O75356 |
| Positive Control | EBV-transformed human B lymphoblastoid cell line |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 70-100 |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest ACE2 was unable to protect EB‐LCL target cells from lysis by LYDMA‐specific cytotoxic T cells in a chromium‐release assay. Recommended Concentrations: FACS: 1µg/5x105 cells Immunofluorescence on acetone-fixed frozen sections or cell smears: 1-2µg/ml Western blot (ECL): 0.2µg/ml Concentration 1mg/ml as standard |
| Research Area | Cardiovascular, Cell Type or Organelle Marker, Immunology, Neurobiology |
References: 2 entries
Rowe et al. 1982. Int J Cancer. 29(4):373-81. PMID: 6282762.
Monoclonal antibodies to Epstein-Barr virus-induced, transformation-associated cell surface antigens: binding patterns and effect upon virus-specific T-cell cytotoxicity.
Europe PMC ID: 6282762
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References: 2 entries
Rowe et al. 1982. Int J Cancer. 29(4):373-81. PMID: 6282762.
Monoclonal antibodies to Epstein-Barr virus-induced, transformation-associated cell surface antigens: binding patterns and effect upon virus-specific T-cell cytotoxicity.
Add a reference
