U-2 OS Gal4-p300 Cell Line
Invented by Dr Yili Yin from University of Dundee
Invented at University of Dundee
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151579 |
| Antigen/Gene or Protein Targets | Gal4-p300 |
| Parental Line | U-2 OS |
| Host | Human |
| Disease Keywords | Osteosarcoma |
| Model | Transgenic |
| Relevance | U2-OS cells expressing Gal4-p300. p300 is a transcriptional coactivator that functions as integrator of numerous signaling pathways and are utilized by many DNA binding proteins to facilitate transcriptional activation. p300 shares numerous conserved domains with CREB binding protein (CBP), which is also a transcriptional coactivator. These shared domains include a histone acetyl transferase (HAT) domain, a bromo domain, and three cysteine- and histidine-rich domains. CBP also interacts with the RNA polymerase II holoenzyme and p300/CBP both contain transcriptional activation domains that function independently of HAT activity. |
| Production Details | U2-OS cells were co-transfected with both the Gal4p300CRD1 expression vector (zeocin selection marker, backbone: pcDNA4/TO) and the Gal4-EIB-luciferase reporter vector (neomycin selection marker, backbone: pCG4 without the CMV promoter) using Fugene 6 transfection reagent.24 hours after transfection, stable transfectants were selected using G418 3.0 mg /ml and Zeocin 3.0 mg/ml until massive cell death, and then grown under G418 0.5 mg /ml and Zeocin 0.5 mg/ml for further selection. Established single Zeocin-resistant and Neomycin-resistant clones were isolated and expanded to generate individual clonal cell lines. These cell lines were verified by testing each clone for induction of high luciferase activity after the depletion of SUMO-conjugating enzyme Ubc9. |
| Research Area | Epigenetics & Nuclear Signalling, Gene Expression |
| Recommended Growing Conditions | DMEM plus 10% Foetal Bovine Serum and 1% Penicillin-Streptomycin |
References: 2 entries
Girdwood et al. 2003. Mol Cell. 11(4):1043-54. PMID: 12718889.
P300 transcriptional repression is mediated by SUMO modification.
Europe PMC ID: 12718889
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References: 2 entries
Girdwood et al. 2003. Mol Cell. 11(4):1043-54. PMID: 12718889.
P300 transcriptional repression is mediated by SUMO modification.
Add a reference
