Anti-CD44v3 [2C5]
Invented at University of Oxford
- Datasheet
- References (14)
- Inventor Info
Info
| Catalogue Number | 151572 |
| Applications | FACS IHC IP WB |
| Antigen/Gene or Protein Targets | CD44v3-10-Fc |
| Synonyms | CD44 Molecule; Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein; Hyaluronate Receptor; Hermes Antigen; ECMR-III; HUTCH-I; Epican; CDW44; MDU2; MDU3; MIC4; LHR; Cell Surface Glycoprotein CD44; Phagocytic Glycoprotein I; Soluble CD44; CSPG8; HCELL; PGP-1; MC56; Pgp1 |
| Reactivity | Human |
| Relevance |
Monoclonal antibody which binds all isoforms of CD44. Background and Research Application CD44H, also called CD44s, refers to the standard form of CD44 without variable exons. A large number of CD44 isoforms can be generated by the insertion of different combinations of at least nine exons. CD44 is an integral cell membrane glycoprotein that binds to hyaluronan and is involved in matrix adhesion, lymphocyte activation, and lymph node homing. The CD44 protein is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, haematopoiesis, and tumour metastasis. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor. |
| Host | Mouse |
| Immunogen | Human chimeric fusion protein |
| Immunogen UniProt ID | P16070 |
| Subclass | IgG2a |
| Recommended Growing Conditions | DMEM + 10% FBS |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody recognizes all CD44 protein isoforms. Concentration 1mg/ml as standard |
| Research Area | Adhesion, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology |
References: 14 entries
Nagano et al. 2004. Cancer Sci. 95(12):930-5. PMID: 15596040.
Lynch et al. 2004. Nat Rev Immunol. 4(12):931-40. PMID: 15573128.
Consequences of regulated pre-mRNA splicing in the immune system.
Europe PMC ID: 15573128
Mechanism and biological significance of CD44 cleavage.
Europe PMC ID: 15596040
Murakami et al. 2003. Oncogene. 22(10):1511-6. PMID: 12629514.
Presenilin-dependent gamma-secretase activity mediates the intramembranous cleavage of CD44.
Europe PMC ID: 12629514
Ponta et al. 2003. Nat Rev Mol Cell Biol. 4(1):33-45. PMID: 12511867.
CD44: from adhesion molecules to signalling regulators.
Europe PMC ID: 12511867
Yu et al. 1996. J Biol Chem. 271(34):20603-7. PMID: 8702806.
A new alternatively spliced exon between v9 and v10 provides a molecular basis for synthesis of soluble CD44.
Europe PMC ID: 8702806
Fox et al. 1994. Cancer Res. 54(16):4539-46. PMID: 7519124.
Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms.
Europe PMC ID: 7519124
Screaton et al. 1992. Proc Natl Acad Sci U S A. 89(24):12160-4. PMID: 1465456.
Genomic structure of DNA encoding the lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons.
Europe PMC ID: 1465456
Add a reference
References: 14 entries
Nagano et al. 2004. Cancer Sci. 95(12):930-5. PMID: 15596040.
Lynch et al. 2004. Nat Rev Immunol. 4(12):931-40. PMID: 15573128.
Consequences of regulated pre-mRNA splicing in the immune system.
Mechanism and biological significance of CD44 cleavage.
Murakami et al. 2003. Oncogene. 22(10):1511-6. PMID: 12629514.
Presenilin-dependent gamma-secretase activity mediates the intramembranous cleavage of CD44.
Ponta et al. 2003. Nat Rev Mol Cell Biol. 4(1):33-45. PMID: 12511867.
CD44: from adhesion molecules to signalling regulators.
Yu et al. 1996. J Biol Chem. 271(34):20603-7. PMID: 8702806.
A new alternatively spliced exon between v9 and v10 provides a molecular basis for synthesis of soluble CD44.
Fox et al. 1994. Cancer Res. 54(16):4539-46. PMID: 7519124.
Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms.
Screaton et al. 1992. Proc Natl Acad Sci U S A. 89(24):12160-4. PMID: 1465456.
Genomic structure of DNA encoding the lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons.
Add a reference
