Anti-XPF [XPF 3F2/3]
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151521 |
| Applications | ELISA IHC IF WB |
| Antigen/Gene or Protein Targets | XPF |
| Reactivity | Human |
| Relevance | Nucleotide excision repair (NER) is a DNA repair pathway that removes lesions induced by a variety of agents such as UV irradiation. ERCC1 and XPF form the heterodimer ERCC1-XPF, forming a DNA endonuclease that is essential for the dual incision step of NER (cleaves 5' of the DNA lesion). |
| Host | Mouse |
| Immunogen | A recombinant His-tagged fragment of human XPF protein produced in E. coli |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes | For a negative control test, embed human cell lines in paraffin and section and stain exactly as one would do for human tissue. It is then possible to include verified XPF-defective cells in the experiment. See references for further details. XP2YO cells are readily available from public cell banks. |
| Research Area | DNA Damage and Repair, Epigenetics & Nuclear Signalling |
References: 4 entries
Facista et al. 2012. Genome Integr. 3(1):3. PMID: 22494821.
Deficient expression of DNA repair enzymes in early progression to sporadic colon cancer.
Europe PMC ID: 22494821
Bhagwat et al. 2009. Cancer Res. 69(17):6831-8. PMID: 19723666.
Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue.
Europe PMC ID: 19723666
Add a reference
References: 4 entries
Facista et al. 2012. Genome Integr. 3(1):3. PMID: 22494821.
Deficient expression of DNA repair enzymes in early progression to sporadic colon cancer.
Bhagwat et al. 2009. Cancer Res. 69(17):6831-8. PMID: 19723666.
Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue.
Add a reference
