DR3 KO Mouse
Invented by Dr Eddie Wang from Cardiff University , Dr Mike Owen from Independent Pharmaceutical And Biotech Consultant
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151474 |
| Antigen/Gene or Protein Targets | DR3 (Death Domain Receptor 3) |
| Relevance | In vivo study of DR3-induced apoptosis & function; in vivo sstudies of negative T cell selection and development. The DR3-/- mouse exhibits complete knockout of DR3, a death domain-containing tumour necrosis factor receptor. which mediates one of the key regulators of the cell division cycle. Negative selection and anti-CD3-induced apoptosis are significantly impaired in DR3-null mice. In contrast, both superantigen-induced negative selection and positive selection are normal. The pre-T-cell receptor-mediated checkpoint, which is dependent on TNFR signaling, is also unaffected in DR3-deficient mice. These data reveal a nonredundant in vivo role for this TNF receptor family member in the removal of self-reactive T cells in the thymus. |
| Production Details | A DR3 targeting vector, replacing the entire DR3 coding region with a loxP flanked resistance cassette, was transfected into GK129 ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele. |
| Conditional | No |
| Growth/Phenotype Keywords | Immune abnormalities (T cell development); |
| Mouse Genetic Background/Cross History | C57BL/6 |
| Zygosity | Homozygous |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, Cell Signaling & Signal Transduction, Genetic Studies Tools, Immunology |
References: 4 entries
Twohig et al. 2010. J Neurosci. 30(10):3782-92. PMID: 20220013.
Age-dependent maintenance of motor control and corticostriatal innervation by death receptor 3.
Europe PMC ID: 20220013
Wang et al. 2001. Mol Cell Biol. 21(10):3451-61. PMID: 11313471.
DR3 regulates negative selection during thymocyte development.
Europe PMC ID: 11313471
Add a reference
References: 4 entries
Twohig et al. 2010. J Neurosci. 30(10):3782-92. PMID: 20220013.
Age-dependent maintenance of motor control and corticostriatal innervation by death receptor 3.
Wang et al. 2001. Mol Cell Biol. 21(10):3451-61. PMID: 11313471.
DR3 regulates negative selection during thymocyte development.
Add a reference
