Anti-Ufd1 [5e2]
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151414 |
| Applications | ELISA IF IP WB |
| Antigen/Gene or Protein Targets | Ufd1 |
| Reactivity | Human, Rat, Mouse and Xenopus laevis |
| Relevance | The Ufd1 protein (human gene: UFD1L) forms a heterodimer with another protein, Npl4. Ufd1-Npl4 serves as an ubiquitin-adapter for the VCP/p97 AAA ATPase. The ATPase complex is required for endoplasmic reticulum-associated degradation and regulation of spindle dynamics, and nuclear envelope formation after mitosis. |
| Host | Mouse |
| Immunogen | Full length mouse Ufd1 expressed in E. coli |
| Positive Control | HeLa or HEK293 cell lysate. |
| Subclass | IgG2a |
| Molecular Weight (kDa) | 40 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 10% FCS. |
| Strain | Balb/c |
| Notes |
Recommended Concentrations: Western Blot: 0.5 ug/ml Immunofluorescence: 5 ug/ml, (formaldehyde-fixed) |
| Research Area | Cancer, Cell Cycle |
References: 6 entries
Cao et al. 2003. Cell. 115(3):355-67. PMID: 14636562.
The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis.
Europe PMC ID: 14636562
Hetzer et al. 2001. Nat Cell Biol. 3(12):1086-91. PMID: 11781570.
Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
Europe PMC ID: 11781570
Meyer et al. 2000. EMBO J. 19(10):2181-92. PMID: 10811609.
A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.
Europe PMC ID: 10811609
Add a reference
References: 6 entries
Cao et al. 2003. Cell. 115(3):355-67. PMID: 14636562.
The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis.
Hetzer et al. 2001. Nat Cell Biol. 3(12):1086-91. PMID: 11781570.
Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.
Meyer et al. 2000. EMBO J. 19(10):2181-92. PMID: 10811609.
A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways.
Add a reference
