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Anti-BCL2 [bcl-2/124]
RECENTLY UPDATED

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151407
Applications IHC WB
Antigen/Gene or Protein Targets Bcl2
Reactivity Human
Relevance
RECENTLY UPDATED
Monoclonal antibody which binds anti-apoptotic regulator BCL2, used to aid in differentiating between various lymphomas and as prognostic biomarker in various cancers.

Background and Research Application
BCL2 is a member of the BCL-2 family of regulatory proteins, involved in apoptosis, which promotes cell survival and inhibits the role of pro-apoptotic proteins such as Bax and Bak. The pro-survival protein BCL2 derives its name from B-cell lymphoma 2. It is the second member of a range of proteins initially described in chromosomal translocations involving chromosomes 14 and 18 in follicular lymphomas. Damage to the Bcl-2 gene has been identified as a possible cause of schizophrenia and autoimmunity, a cause of cancers, including melanoma, breast, prostate, chronic lymphocytic leukaemia, and lung cancer. It also provides resistance to cancer treatments .
This antibody was created to see if the BCL2 protein is a specific marker to the 14;18 chromosomal translocation in human follicular lymphoma. Previous studies claimed the BCL2 protein was not found in normal tissue but was present in all lymphomas where this specific translocation was observed. This antibody helped show the immunohistologic demonstration of BCL2 isn’t specific for the presence of 14;18 translocation.
Host Mouse
Immunogen Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
Immunogen UniProt ID
RECENTLY UPDATED
P10415
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-BCL2 can be used to differentiate between lymphomas which express BCL2 and the small proportion of neoplasms which are BCL2 negative, and has been used as a predictive biomarker for recurrence of radical prostatectomy for prostate cancer.
Anti-BCL2 labels neoplastic lymphoid follicles. In the case of follicular lymphoma, its value is to distinguish between reactive and neoplastic follicular proliferation in lymph node biopsies. It can also help differentiate between follicular lymphoma and diffuse large cell lymphomas, and between follicular lymphomas and reactive lymph nodes.


Concentration
1mg/ml as standard
Research Area
RECENTLY UPDATED
Apoptosis and Programmed Cell Death, Cancer, Cell Signaling & Signal Transduction, Metabolism

References

There are 47 reference entries for this reagent.

View All References

References: 47 entries
RECENTLY UPDATED

Shimono et al. 2018. Am J Surg Pathol. 42(11):1466-1471. PMID: 30307409.

Nanni et al. 2018. Mol Cell Biol. 38(13):. PMID: 29685904.

Péricart et al. 2017. Medicine (Baltimore). 96(47):e8705. PMID: 29381956.

Chang et al. 2016. J Formos Med Assoc. :. PMID: 27773559.

Immunophenotypic and genetic characteristics of diffuse large B-cell lymphoma in Taiwan.

Europe PMC ID: 27773559

Ishiguro et al. 2016. Hum Cell. :. PMID: 27613543.

IHC

Molecular cytogenetic characterization of two established ESFT cell lines.

Europe PMC ID: 27613543

Otali et al. 2016. Biotech Histochem. :1-18. PMID: 27149658.

IHC

A standard tissue as a control for histochemical and immunohistochemical staining.

Europe PMC ID: 27149658

Cortese et al. 2016. Neurology. :. PMID: 26843560.

Cutaneous adverse events in multiple sclerosis patients treated with daclizumab.

Europe PMC ID: 26843560

Miltiades et al. 2015. Clin Cancer Res. :. PMID: 26700206.

The Stat3/5 Signaling Biosignature in Hematopoietic Stem/Progenitor Cells Predicts Response and Outcome in Myelodysplastic Syndrome Patients Treated with Azacitidine.

Europe PMC ID: 26700206

Tajima et al. 2015. Int J Clin Exp Pathol. 8(3):3262-9. PMID: 26045850.

IHC

Pankova et al. 2015. Tumour Biol. :. PMID: 26456960.

IHC

Recurrence of squamous cell lung carcinoma is associated with the co-presence of reactive lesions in tumor-adjacent bronchial epithelium.

Europe PMC ID: 26456960

Miyagi Maeshima et al. 2015. J Clin Exp Hematop. 55(1):7-11. PMID: 26106000.

IHC

Primary synovial sarcoma of the right heart involving the tricuspid valve in an elderly Chinese woman: a case report.

Europe PMC ID: 26112006

Huo et al. 2015. Diagn Pathol. 10:80. PMID: 26112006.

Higashi et al. 2015. Leuk Lymphoma. :1-6. PMID: 25860238.

Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

Europe PMC ID: 25860238

Cystic synovial sarcoma of the pleura mimicking a cystic thymoma: a case report illustrating the role of decreased INI-1 expression in differential diagnosis.

Europe PMC ID: 26045850

Histopathological Characteristics of Lymphomas in the Upper Aerodigestive Tract. A Single-Institute Study in Japan.

Europe PMC ID: 26106000

Navratil et al. 1995. J Clin Pathol. 48(1):18-21. PMID: 7706514.

Ashton-Key et al. 1995. Histopathology. 26(1):75-8. PMID: 7713486.

Heterogeneity of bcl-2 expression in MALT lymphoma.

Europe PMC ID: 7713486

Expression of the bcl-2 protein in B cell lymphomas arising from mucosa associated lymphoid tissue.

Europe PMC ID: 7706514

Piris et al. 1994. Br J Cancer. 69(2):337-41. PMID: 8297731.

p53 and bcl-2 expression in high-grade B-cell lymphomas: correlation with survival time.

Europe PMC ID: 8297731

Leek et al. 1994. Br J Cancer. 69(1):135-9. PMID: 8286195.

de Jong et al. 1994. Cancer Res. 54(1):256-60. PMID: 8261449.

Subcellular localization of the bcl-2 protein in malignant and normal lymphoid cells.

Europe PMC ID: 8261449

bcl-2 in normal human breast and carcinoma, association with oestrogen receptor-positive, epidermal growth factor receptor-negative tumours and in situ cancer.

Europe PMC ID: 8286195

Pezzella et al. 1993. N Engl J Med. 329(10):690-4. PMID: 8393963.

bcl-2 protein in non-small-cell lung carcinoma.

Europe PMC ID: 8393963

Doussis et al. 1993. Am J Clin Pathol. 99(6):663-7. PMID: 8322700.

An immunocytochemical study of p53 and bcl-2 protein expression in Hodgkin's disease.

Europe PMC ID: 8322700

Pezzella et al. 1993. Histopathology. 22(1):39-44. PMID: 8436340.

Immunohistochemical detection of p53 and bcl-2 proteins in non-Hodgkin's lymphoma.

Europe PMC ID: 8436340

Gaulard et al. 1992. Am J Pathol. 140(5):1089-95. PMID: 1374590.

Expression of the bcl-2 gene product in follicular lymphoma.

Europe PMC ID: 1374590

Pezzella et al. 1992. Br J Cancer. 65(1):87-9. PMID: 1733447.

Evaluation of bcl-2 protein expression and 14;18 translocation as prognostic markers in follicular lymphoma.

Europe PMC ID: 1733447

Pezzella et al. 1990. Lancet. 336(8729):1510-1. PMID: 1979117.

Bcl-2 protein expression in follicular lymphomas in absence of 14;18 translocation.

Europe PMC ID: 1979117

Lehmkuhl et al. 1977. Am J Vet Res. 38(11):1717-20. PMID: 201196.

Investigation of causative agents of bovine respiratory tract disease in a beef cow-calf herd with an early weaning program.

Europe PMC ID: 201196


Add a reference

References: 47 entries
RECENTLY UPDATED

Shimono et al. 2018. Am J Surg Pathol. 42(11):1466-1471. PMID: 30307409.

Nanni et al. 2018. Mol Cell Biol. 38(13):. PMID: 29685904.

Péricart et al. 2017. Medicine (Baltimore). 96(47):e8705. PMID: 29381956.

Chang et al. 2016. J Formos Med Assoc. :. PMID: 27773559.

Immunophenotypic and genetic characteristics of diffuse large B-cell lymphoma in Taiwan.

Ishiguro et al. 2016. Hum Cell. :. PMID: 27613543.

IHC

Molecular cytogenetic characterization of two established ESFT cell lines.

Otali et al. 2016. Biotech Histochem. :1-18. PMID: 27149658.

IHC

A standard tissue as a control for histochemical and immunohistochemical staining.

Cortese et al. 2016. Neurology. :. PMID: 26843560.

Cutaneous adverse events in multiple sclerosis patients treated with daclizumab.

Miltiades et al. 2015. Clin Cancer Res. :. PMID: 26700206.

The Stat3/5 Signaling Biosignature in Hematopoietic Stem/Progenitor Cells Predicts Response and Outcome in Myelodysplastic Syndrome Patients Treated with Azacitidine.

Tajima et al. 2015. Int J Clin Exp Pathol. 8(3):3262-9. PMID: 26045850.

IHC

Pankova et al. 2015. Tumour Biol. :. PMID: 26456960.

IHC

Recurrence of squamous cell lung carcinoma is associated with the co-presence of reactive lesions in tumor-adjacent bronchial epithelium.

Miyagi Maeshima et al. 2015. J Clin Exp Hematop. 55(1):7-11. PMID: 26106000.

IHC

Primary synovial sarcoma of the right heart involving the tricuspid valve in an elderly Chinese woman: a case report.

Huo et al. 2015. Diagn Pathol. 10:80. PMID: 26112006.

Higashi et al. 2015. Leuk Lymphoma. :1-6. PMID: 25860238.

Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

Cystic synovial sarcoma of the pleura mimicking a cystic thymoma: a case report illustrating the role of decreased INI-1 expression in differential diagnosis.

Histopathological Characteristics of Lymphomas in the Upper Aerodigestive Tract. A Single-Institute Study in Japan.

Navratil et al. 1995. J Clin Pathol. 48(1):18-21. PMID: 7706514.

Ashton-Key et al. 1995. Histopathology. 26(1):75-8. PMID: 7713486.

Heterogeneity of bcl-2 expression in MALT lymphoma.

Expression of the bcl-2 protein in B cell lymphomas arising from mucosa associated lymphoid tissue.

Piris et al. 1994. Br J Cancer. 69(2):337-41. PMID: 8297731.

p53 and bcl-2 expression in high-grade B-cell lymphomas: correlation with survival time.

Leek et al. 1994. Br J Cancer. 69(1):135-9. PMID: 8286195.

de Jong et al. 1994. Cancer Res. 54(1):256-60. PMID: 8261449.

Subcellular localization of the bcl-2 protein in malignant and normal lymphoid cells.

bcl-2 in normal human breast and carcinoma, association with oestrogen receptor-positive, epidermal growth factor receptor-negative tumours and in situ cancer.

Pezzella et al. 1993. N Engl J Med. 329(10):690-4. PMID: 8393963.

bcl-2 protein in non-small-cell lung carcinoma.

Doussis et al. 1993. Am J Clin Pathol. 99(6):663-7. PMID: 8322700.

An immunocytochemical study of p53 and bcl-2 protein expression in Hodgkin's disease.

Pezzella et al. 1993. Histopathology. 22(1):39-44. PMID: 8436340.

Immunohistochemical detection of p53 and bcl-2 proteins in non-Hodgkin's lymphoma.

Gaulard et al. 1992. Am J Pathol. 140(5):1089-95. PMID: 1374590.

Expression of the bcl-2 gene product in follicular lymphoma.

Pezzella et al. 1992. Br J Cancer. 65(1):87-9. PMID: 1733447.

Evaluation of bcl-2 protein expression and 14;18 translocation as prognostic markers in follicular lymphoma.

Pezzella et al. 1990. Lancet. 336(8729):1510-1. PMID: 1979117.

Bcl-2 protein expression in follicular lymphomas in absence of 14;18 translocation.

Lehmkuhl et al. 1977. Am J Vet Res. 38(11):1717-20. PMID: 201196.

Investigation of causative agents of bovine respiratory tract disease in a beef cow-calf herd with an early weaning program.


Add a reference