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Anti-PECAM1 [JC70]

Info

Catalogue Number 151357
Applications FACS IHC IF WB
Antigen/Gene or Protein Targets PECAM1 (CD31)
Synonyms Platelet/Endothelial Cell Adhesion Molecule 1; CD31 Antigen; EndoCAM; PECAM-1; GPIIA; PECA1; CD31/EndoCAM; CD31
Reactivity Human
Relevance PECAM1 (CD31) is a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, particular T-cell subsets, and is also a major constituent of the endothelial cell intercellular junction. PECAM1 is implicated in several functions, including transendothelial migration of leukocytes, angiogenesis, and integrin activation. JC70 is a good marker of endothelial cells in paraffin sections.
Host Mouse
Immunogen Cell membrane preparation from the spleen of a patient with hairy cell leukaemia
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM)
Notes Ultroser G can be used at 1% if the cells are not growing well.
Immunocytochemistry (ICC) 1:10-1:100
Immunofluorescence (IF) 1:10-1:100
Immunohistochemistry (Paraffin) (IHC (P)) 1:100
Flow Cytometry (FACS) 1:2
Western Blot (WB) 1:1-1:100
Research Area Adhesion, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Stem Cell Biology

References: 7 entries

Muller WA, Kishimoto T, et al (eds) 1997. Leucocyte Typing VI, Garland Publishing Inc., New York and London, p 362-364. ISBN-13: 978-0815327455

Woei-A-Jin et al. 2016. Br J Cancer. :. PMID: 27404454.

IHC

Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.

IHC

Zhao et al. 2013. Cancer Res. 73(20):6149-63. PMID: 24097821.

Ding et al. 2013. PLoS One. 8(5):e63628. PMID: 23675495.

Orecchia et al. 2011. PLoS One. 6(9):e24307. PMID: 21931678.

Parums et al. 1990. J Clin Pathol. 43(9):752-7. PMID: 2212067.


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