Anti-SIGLEC2 [4KB128]
Invented by Karen Pulford from University of Oxford
Invented at University of Oxford
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151339 |
| Applications | FACS |
| Antigen/Gene or Protein Targets | Sialic acid binding Ig-like lectin 2 (SIGLEC2, CD22, BL-CAM) |
| Reactivity | Human |
| Relevance | SIGLEC-2 is first expressed in the cytomplasm of pre- and pro- B cells. SIGLEC-2 is broadly expressed in normal and neoplastic B cells and absent from other leucocytes and tissues. SIGLEC-2 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. SIGLEC-2 is strongly expressed in hairy cell leukaemias. |
| Host | Mouse |
| Immunogen | B cell lymphoma cells |
| Subclass | IgG1 kappa |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Notes | Ultroser G can be used at 1% if the cells are not growing well. |
| Research Area | Cancer, Immunology, Stem Cell Biology |
References: 6 entries
Law et al. 1994. Immunol Today. 15(9):442-9. PMID: 7945784.
Regulation of lymphocyte activation by the cell-surface molecule CD22.
Europe PMC ID: 7945784
Mason et al. 1987. Blood. 69(3):836-40. PMID: 3101766.
Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells.
Europe PMC ID: 3101766
Campana et al. 1985. J Immunol. 134(3):1524-30. PMID: 3918103.
Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue.
Europe PMC ID: 3918103
Add a reference
References: 6 entries
Law et al. 1994. Immunol Today. 15(9):442-9. PMID: 7945784.
Regulation of lymphocyte activation by the cell-surface molecule CD22.
Mason et al. 1987. Blood. 69(3):836-40. PMID: 3101766.
Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells.
Campana et al. 1985. J Immunol. 134(3):1524-30. PMID: 3918103.
Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue.
Add a reference
