Anti-CyclinA [E67.1]
Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (15)
- Inventor Info
Info
| Catalogue Number | 151303 |
| Applications | FACS IHC IF IP WB |
| Antigen/Gene or Protein Targets | Cyclin A |
| Synonyms |
CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2 Human Entrez Gene ID: 890 Human SwissProt: P20248 Human Unigene: 58974 Human Gene Symbol: CCNA2 Human Chromosome Location: 4q27 |
| Reactivity | Bovine, Human, Mink and Mouse |
| Relevance |
Monoclonal antibody capable of immunoprecipitating active CDK/Cyclin A H1 kinase. Background and Research Application The anti-Cyclin A2, clone E67.1, recognizes a protein of 54kDa, which is identified as cyclin A. Its epitope is located on amino acids 144-148 of human Cyclin A2. Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs), controlling transition at various specific phases of the cell cycle. Cyclin A is involved in the regulation of the cell cycle and is essential for progression through the S-phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S-phase, with passage through to the G2 phase. E67.1 is one of the only antibodies capable of immunoprecipitating active CDK/Cyclin A H1 kinase from such a wide range of species. This antibody binds to an epitope within amino acids 144-148 of human Cyclin A2, mapped out by phage selection. |
| Host | Mouse |
| Immunogen | Full length bovine Cyclin A protein |
| Immunogen UniProt ID | P78396 |
| Positive Control | K-562 and HeLa Cells |
| Subclass | IgG2a kappa |
| Molecular Weight (kDa) | 54kDa |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Cellular Localization: Predominantly nuclear Suggested dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Concentration 1mg/ml as standard |
| Research Area | Cancer, Cell Cycle, Epigenetics & Nuclear Signalling |
References: 15 entries
Jones et al. 2018. J Cell Biol. 217(9):3203-3218. PMID: 29930204.
Roilo et al. 2018. Nucleic Acids Res. 46(6):3198-3210. PMID: 29361038.
Whalley et al. 2015. Nat Commun. 6:7437. PMID: 26078008.
Ge et al. 2010. J Cell Biol. 191(7):1285-97. PMID: 21173116.
Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories.
Europe PMC ID: 21173116
Ho et al. 2008. J Biol Chem. 283(9):5477-85. PMID: 18077453.
Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network.
Europe PMC ID: 18077453
Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
Europe PMC ID: 11120603
Sandhu et al. 2000. Oncogene. 19(47):5314-23. PMID: 11103932.
Reduction of Cdc25A contributes to cyclin E1-Cdk2 inhibition at senescence in human mammary epithelial cells.
Europe PMC ID: 11103932
Slingerland et al. 1994. Mol Cell Biol. 14(6):3683-94. PMID: 8196612.
A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells.
Europe PMC ID: 8196612
Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
Simian virus 40 large T antigen associates with cyclin A and p33cdk2.
Europe PMC ID: 8411358
Add a reference
References: 15 entries
Jones et al. 2018. J Cell Biol. 217(9):3203-3218. PMID: 29930204.
Roilo et al. 2018. Nucleic Acids Res. 46(6):3198-3210. PMID: 29361038.
Whalley et al. 2015. Nat Commun. 6:7437. PMID: 26078008.
Ge et al. 2010. J Cell Biol. 191(7):1285-97. PMID: 21173116.
Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories.
Ho et al. 2008. J Biol Chem. 283(9):5477-85. PMID: 18077453.
Identification of cyclin A2 as the downstream effector of the nuclear phosphatidylinositol 4,5-bisphosphate signaling network.
Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
Sandhu et al. 2000. Oncogene. 19(47):5314-23. PMID: 11103932.
Reduction of Cdc25A contributes to cyclin E1-Cdk2 inhibition at senescence in human mammary epithelial cells.
Slingerland et al. 1994. Mol Cell Biol. 14(6):3683-94. PMID: 8196612.
A novel inhibitor of cyclin-Cdk activity detected in transforming growth factor beta-arrested epithelial cells.
Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
Simian virus 40 large T antigen associates with cyclin A and p33cdk2.
Add a reference
