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Anti-Rad51B [rad51b 1h3/13]


Catalogue Number 151268
Applications IP WB
Antigen/Gene or Protein Targets Rad51B
Reactivity Human
Relevance RAD51B is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51B is also a component for the homologous recombination pathway.
Host Mouse
Immunogen Rad51b 1h3/13 was raised against His-tagged human Rad51B, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-PAGE.
Subclass IgG2a
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Rad51b 1h3/13 detects as little as 10ng of purified protein
Research Area DNA Damage and Repair, Epigenetics & Nuclear Signalling

References: 3 entries

Chun et al. 2013. Mol Cell Biol. 33(2):387-95. PMID: 23149936.

Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.

Albala et al. 1997. Genomics. 46(3):476-9. PMID: 9441753.

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