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Catalogue Number 151196
Applications IHC IP WB
Antigen/Gene or Protein Targets Rad51
Reactivity Bovine, Human and Pig
Relevance Monoclonal antibody which binds RAD51 recombinase protein, associated with DNA repair pathway.

Background and Research Application
RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51 catalyses the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Mutations in the gene can results in breast cancer, mirror movements 2 and Fanconi anaemia.
Host Mouse
Immunogen Full length recombinant human RAD51 protein
Immunogen UniProt ID Q06609
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-RAD51 antibody has been used to immunohistochemically stain biopsy and resected specimens of oesophagus and squamous cell carcinoma of the oesophagus.

Concentration
1mg/ml as standard
Research Area DNA Damage and Repair, Epigenetics & Nuclear Signalling

References

There are 9 reference entries for this reagent.

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References: 9 entries

Vohhodina et al. 2017. Nucleic Acids Res. 45(22):12816-12833. PMID: 29112714.

Subramanyam et al. 2016. Proc Natl Acad Sci U S A. 113(41):E6045-E6054. PMID: 27671650.

Marampon et al. 2016. Oncotarget. 7(5):5383-400. PMID: 26689991.

Ivanov et al. 2003. J Cell Sci. 116(Pt 20):4095-106. PMID: 12953071.

Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks.

Europe PMC ID: 12953071

Masson et al. 1999. EMBO J. 18(22):6552-60. PMID: 10562567.

The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.

Europe PMC ID: 10562567

Barlow et al. 1997. EMBO J. 16(17):5207-15. PMID: 9311981.

Distribution of the Rad51 recombinase in human and mouse spermatocytes.

Europe PMC ID: 9311981


Add a reference

References: 9 entries

Vohhodina et al. 2017. Nucleic Acids Res. 45(22):12816-12833. PMID: 29112714.

Subramanyam et al. 2016. Proc Natl Acad Sci U S A. 113(41):E6045-E6054. PMID: 27671650.

Marampon et al. 2016. Oncotarget. 7(5):5383-400. PMID: 26689991.

Ivanov et al. 2003. J Cell Sci. 116(Pt 20):4095-106. PMID: 12953071.

Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks.

Masson et al. 1999. EMBO J. 18(22):6552-60. PMID: 10562567.

The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.

Barlow et al. 1997. EMBO J. 16(17):5207-15. PMID: 9311981.

Distribution of the Rad51 recombinase in human and mouse spermatocytes.


Add a reference