Anti-CyclinB1 [V92.1]
Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151180 |
| Applications | FACS IHC IF IP WB |
| Antigen/Gene or Protein Targets | Cyclin B1 |
| Synonyms |
CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1 Human Entrez Gene ID 891 Human SwissProt P14635 Human Unigene 23960 Human Gene Symbol CCNB1 Human Chromosome Location 5q13.2 |
| Reactivity | Hamster, Human and Mouse |
| Relevance | Clone V92.1, recognizes a protein of 55-62kDa, identified as cyclin B1. Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin B1 is a marker of cell proliferation and a key component of the cell cycle progression machinery. Cyclin B1 may prove to be a prognostic marker in neoplasia. V92.1 may be used to immunoprecipitate active CDK1/cyclin complexes. |
| Host | Mouse |
| Immunogen | Hamster Cyclin B1 |
| Positive Control | Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma. |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 55-62kDa |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Notes |
Cellular Localization: Cytoplasmic Suggwested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunoprecipitation (1-2ug/500ug protein) (precipitates active CDK1/cyclin B1 complexes); |
| Research Area | Cancer, Cell Cycle, Epigenetics & Nuclear Signalling |
References: 4 entries
Garner et al. 2002. Oncogene. 21(53):8089-104. PMID: 12444545.
Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint.
Europe PMC ID: 12444545
Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
Europe PMC ID: 11120603
Add a reference
References: 4 entries
Garner et al. 2002. Oncogene. 21(53):8089-104. PMID: 12444545.
Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint.
Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.
Add a reference
