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Anti-CD3, Recombinant [UCH-T1®]
Invented by Prof Peter Beverley at Absolute Antibody Cancer Research UK London Research Institute: Lincoln's Inn Fields
Anti-CD3 [UCH-T1®]
Invented by Prof Peter Beverley from Imperial College
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (21)
- Inventor Info
Info
| Catalogue Number | 151175 |
| Applications | FACS IHC IF IP Fn RIA WB |
| Antigen/Gene or Protein Targets | CD3 |
| Synonyms | T3 complex |
| Reactivity | Human |
| Relevance |
CRT trademarked famous anti-CD3 monoclonal antibody, capable of differentiating between T vs B cells lymphomas and leukaemia’s. Background and Research Application The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3γ, CD3δ and 2X CD3Ɛ), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia’s. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemia’s and approximately 70% of acute lymphoblastic leukaemia’s of T cell origin. It can also be used to study the role of CD3 in TCR signal transduction events. This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunised against human lymphocytes, with UCHT1 being one of the first successful fusions. |
| Host | Mouse |
| Immunogen | Human infant thymocytes and Sezary cells. |
| Immunogen UniProt ID | P04234 |
| Subclass | IgG1 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 5% FCS. |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Recommended for the enrichment of T cell populations by cell sorting or panning. UCHT1 binds to an acidic region of CD3-epsilon, occluding this region form direct interaction with the T cell receptor. UCHT1 readily detects T-cell populations, in peripheral blood and lymph nodes, as CD3 is present on around 68-82% of healthy peripheral blood lymphocytes. Concentration 1mg/ml as standard Dilutions for Flow Cyt 4ul for 10^6 cells |
| Research Area | Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology |
References: 21 entries
Bencsikova et al. 2019. BMC Cancer. 19(1):687. PMID: 31307428.
Ollé Hurtado et al. 2019. PLoS One. 14(8):e0216373. PMID: 31398192.
Acquaviva et al. 2019. Front Immunol. 10:1922. PMID: 31474991.
Érsek et al. 2012. J Immunol. 189(4):1602-10. PMID: 22798681.
CD3ζ-chain expression of human T lymphocytes is regulated by TNF via Src-like adaptor protein-dependent proteasomal degradation.
Europe PMC ID: 22798681
Muhammad et al. 2009. J Immunol. 182(12):7672-80. PMID: 19494291.
Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome.
Europe PMC ID: 19494291
Nishi et al. 2008. Glycobiology. 18(12):1065-73. PMID: 18796645.
Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1.
Europe PMC ID: 18796645
Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.
Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.
Europe PMC ID: 11694532
Lafont et al. 1999. J Biol Chem. 274(36):25743-8. PMID: 10464312.
Fn
Evidence for a p21(ras)/Raf-1/MEK-1/ERK-2-independent pathway in stimulation of IL-2 gene transcription in human primary T lymphocytes.
Europe PMC ID: 10464312
Salmerón et al. 1991. J Immunol. 147(9):3047-52. PMID: 1717585.
IP
A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.
Europe PMC ID: 1717585
Kanellopoulos et al. 1983. EMBO J. 2(10):1807-14. PMID: 6227478.
Biosynthesis and molecular nature of the T3 antigen of human T lymphocytes.
Europe PMC ID: 6227478
Garson et al. 1982. Nature. 298(5872):375-7. PMID: 6178042.
Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species.
Europe PMC ID: 6178042
Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.
Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.
Europe PMC ID: 6788570
Add a reference
References: 21 entries
Bencsikova et al. 2019. BMC Cancer. 19(1):687. PMID: 31307428.
Ollé Hurtado et al. 2019. PLoS One. 14(8):e0216373. PMID: 31398192.
Acquaviva et al. 2019. Front Immunol. 10:1922. PMID: 31474991.
Érsek et al. 2012. J Immunol. 189(4):1602-10. PMID: 22798681.
CD3ζ-chain expression of human T lymphocytes is regulated by TNF via Src-like adaptor protein-dependent proteasomal degradation.
Muhammad et al. 2009. J Immunol. 182(12):7672-80. PMID: 19494291.
Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome.
Nishi et al. 2008. Glycobiology. 18(12):1065-73. PMID: 18796645.
Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1.
Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.
Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.
Lafont et al. 1999. J Biol Chem. 274(36):25743-8. PMID: 10464312.
Fn
Evidence for a p21(ras)/Raf-1/MEK-1/ERK-2-independent pathway in stimulation of IL-2 gene transcription in human primary T lymphocytes.
Salmerón et al. 1991. J Immunol. 147(9):3047-52. PMID: 1717585.
IP
A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.
Kanellopoulos et al. 1983. EMBO J. 2(10):1807-14. PMID: 6227478.
Biosynthesis and molecular nature of the T3 antigen of human T lymphocytes.
Garson et al. 1982. Nature. 298(5872):375-7. PMID: 6178042.
Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species.
Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.
Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.
Add a reference
