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Anti-Involucrin, Recombinant [SY5]
Invented at Absolute Antibody Cancer Research UK London Research Institute: Lincoln's Inn Fields
Anti-Involucrin [SY5]
Invented by Prof Fiona Watt from King's College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (18)
- Inventor Info
Info
| Catalogue Number | 151163 |
| Applications | FACS IHC IF IP WB |
| Antigen/Gene or Protein Targets | Involucrin |
| Reactivity | Dog, Human, Pig and Primate |
| Relevance | Clone SY5 recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it.Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. SY5 is useful for studies of changes in involucrin expression in pathological studies. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. |
| Host | Mouse |
| Immunogen | Purified involucrin from human keratinocytes. |
| Positive Control | MCF-7 cells. Localized to upper spinous and granular layers in normal skin. |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 66-170 |
| Myeloma Used | P3X63Ag8 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
There is weak reactivity with Canine, but it does not react with Mouse. Cellular Localization: Cytoplasmic Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37°C)(Note than enzyme digestion is better than citrate buffer based epitope unmasking) |
| Research Area | Cancer, Cell Signaling & Signal Transduction |
References: 18 entries
Zhong et al. 2016. Cell. 167(1):187-202.e17. PMID: 27662089.
IF
Germline NLRP1 Mutations Cause Skin Inflammatory and Cancer Susceptibility Syndromes via Inflammasome Activation.
Europe PMC ID: 27662089
Morales et al. 2016. Toxicol In Vitro. 36:89-96. PMID: 27448499.
Evaluation of fibrin-based dermal-epidermal organotypic cultures for in vitro skin corrosion and irritation testing of chemicals according to OECD TG 431 and 439.
Europe PMC ID: 27448499
A fundamental investigation into aspects of the physiology and biochemistry of the stratum corneum in subjects with sensitive skin.
Europe PMC ID: 27079667
Raj et al. 2016. Int J Cosmet Sci. :. PMID: 27079667.
Golinski et al. 2015. Cells Tissues Organs. : PMID: 26303436.
IHC
Ravindran et al. 2015. Head Neck. 37(7):982-93. PMID: 24700702.
IHC
Association of differential β-catenin expression with Oct-4 and Nanog in oral squamous cell carcinoma and their correlation with clinicopathological factors and prognosis.
Europe PMC ID: 24700702
Development and Characterization of an Engraftable Tissue-Cultured Skin Autograft: Alternative Treatment for Severe Electrical Injuries.
Europe PMC ID: 26303436
Creppe et al. 2012. Mol Cell Biol. 32(8):1442-52. PMID: 22331466.
MacroH2A1 regulates the balance between self-renewal and differentiation commitment in embryonic and adult stem cells.
Europe PMC ID: 22331466
White et al. 2010. J Immunol. 185(8):4769-76. PMID: 20861360.
Lymphotoxin signals from positively selected thymocytes regulate the terminal differentiation of medullary thymic epithelial cells.
Europe PMC ID: 20861360
Skoog et al. 2009. J Invest Dermatol. 129(1):119-30. PMID: 18633436.
Matrix metalloproteinase-21 expression is associated with keratinocyte differentiation and upregulated by retinoic acid in HaCaT cells.
Europe PMC ID: 18633436
Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.
Characterisation of eight monoclonal antibodies to involucrin.
Europe PMC ID: 1500072
Add a reference
References: 18 entries
Zhong et al. 2016. Cell. 167(1):187-202.e17. PMID: 27662089.
IF
Germline NLRP1 Mutations Cause Skin Inflammatory and Cancer Susceptibility Syndromes via Inflammasome Activation.
Morales et al. 2016. Toxicol In Vitro. 36:89-96. PMID: 27448499.
Evaluation of fibrin-based dermal-epidermal organotypic cultures for in vitro skin corrosion and irritation testing of chemicals according to OECD TG 431 and 439.
A fundamental investigation into aspects of the physiology and biochemistry of the stratum corneum in subjects with sensitive skin.
Raj et al. 2016. Int J Cosmet Sci. :. PMID: 27079667.
Golinski et al. 2015. Cells Tissues Organs. : PMID: 26303436.
IHC
Ravindran et al. 2015. Head Neck. 37(7):982-93. PMID: 24700702.
IHC
Association of differential β-catenin expression with Oct-4 and Nanog in oral squamous cell carcinoma and their correlation with clinicopathological factors and prognosis.
Development and Characterization of an Engraftable Tissue-Cultured Skin Autograft: Alternative Treatment for Severe Electrical Injuries.
Creppe et al. 2012. Mol Cell Biol. 32(8):1442-52. PMID: 22331466.
MacroH2A1 regulates the balance between self-renewal and differentiation commitment in embryonic and adult stem cells.
White et al. 2010. J Immunol. 185(8):4769-76. PMID: 20861360.
Lymphotoxin signals from positively selected thymocytes regulate the terminal differentiation of medullary thymic epithelial cells.
Skoog et al. 2009. J Invest Dermatol. 129(1):119-30. PMID: 18633436.
Matrix metalloproteinase-21 expression is associated with keratinocyte differentiation and upregulated by retinoic acid in HaCaT cells.
Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.
Characterisation of eight monoclonal antibodies to involucrin.
Add a reference
