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Monoclonal Antibodies

Anti-Integrin a3 [IA3]

Invented by Prof Joyce Taylor-Papadimitriou from King's College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Seltmann K, Cheng F, Wiche G, Eriksson JE, Magin TM. Keratins Stabilize Hemidesmosomes through Regulation of β4-Integrin Turnover. J Invest Dermatol. 2015 Jun;135(6):1609-1620. doi: 10.1038/jid.2015.46. Epub 2015 Feb 10. PMID: 25668239.. Absence of keratins leads to higher turnover and reduced surface level of β4-integrin. (a) FRAP analysis of β4-integrin dynamics with or without inhibitors of MEK1/2 and EGFR. Error bars correspond to five independent experiments with each comprising at least 30 cells. (b) Western blot analysis of Erk1/2 phosphorylation of total WT and KO lysates treated with or without EGFR and MEK1/2 inhibitors. (c) Flow-cytometric profile of integrin surface expression. Red peak––WT, black––KO, and green/blue––K5 or K6 rescue cells. (d) Keratinocytes were starved and then triggered with EGF for 15 minutes. (e) Cells were treated with indicated kinase inhibitors. Control cells are illustrated by red and black peaks (WT and KO), whereas green and blue display inhibited WT and KO cells. All experiments were repeated at least three times (n=3). ***P< 0.01.
Adapted from Stawikowski MJ, Aukszi B, Stawikowska R, Cudic M, Fields GB. Glycosylation modulates melanoma cell α2β1 and α3β1 integrin interactions with type IV collagen. J Biol Chem. 2014 Aug 1;289(31):21591-604. doi: 10.1074/jbc.M114.572073. Epub 2014 Jun 23. PMID: 24958723; PMCID: PMC4118119. The cell surface concentrations of the α2 and α3 subunits of the α2β1 and α3β1 integrins evaluated for melanoma cell lines by immunocytochemistry. A, image analysis for each cell line for both receptor subunits and IgG background. Bars indicate 100 μm. B, semiquantitative numerical values for cellular integrin concentrations, obtained by relative fluorescence intensities of the same number of cells, normalized by area measured and by subtraction of the autofluorescence of the cells.
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Catalogue Number 151109
Applications FACS IHC IF Fn
Antigen/Gene or Protein Targets Integrin a3 (CD49c)
Reactivity Human
Relevance Monoclonal antibody with use studying inhibition of cellular adhesion.

Background and Research Application
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin a3 is found on most cell types. Integrin a3 associates with b1 integrin to form integrin a3b1, which is one of the most abundant keratinocyte integrins and binds laminin 5.
IA3 is useful for studies on inhibition of cell-cell adhesion and cell-substrate adhesion.
Host Mouse
Immunogen Transfected human breast epithelial cell line MTSV1-7, hygromycin resistant.
Immunogen UniProt ID P26006
Subclass IgG1
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Concentration
1mg/ml as standard
Research Area Adhesion, Cancer

References

There are 13 reference entries for this reagent.

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References: 13 entries

Tremblay et al. 2017. Sci Rep. 7:46574. PMID: 28422167.

View  

Tremblay et al. 2017. Sci Rep. 7:46574. PMID: 28422167.

View  

Seltmann et al. 2015. J Invest Dermatol. 135(6):1609-1620. PMID: 25668239.

View  

Seltmann et al. 2015. J Invest Dermatol. 135(6):1609-20. PMID: 25668239.

FACS IF

View  

Keratins Stabilize Hemidesmosomes through Regulation of β4-Integrin Turnover.

Europe PMC ID: 25668239

View  

Stawikowski et al. 2014. J Biol Chem. 289(31):21591-604. PMID: 24958723.

IF

View  

Glycosylation modulates melanoma cell α2β1 and α3β1 integrin interactions with type IV collagen.

Europe PMC ID: 24958723

View  

Pytlík et al. 2009. Biomaterials. 30(20):3415-27. PMID: 19362364.

FACS

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The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.

Europe PMC ID: 19362364

View  

Turner et al. 2006. Circulation. 114(8):820-9. PMID: 16908762.

View  

Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study.

Europe PMC ID: 16908762

View  

Berdichevsky et al. 1994. J Cell Sci. 107 ( Pt 12):3557-68. PMID: 7535787.

View  

Branching morphogenesis of human mammary epithelial cells in collagen gels.

Europe PMC ID: 7535787

View  

Add a reference

References: 13 entries

Tremblay et al. 2017. Sci Rep. 7:46574. PMID: 28422167.

View  

Tremblay et al. 2017. Sci Rep. 7:46574. PMID: 28422167.

View  

Seltmann et al. 2015. J Invest Dermatol. 135(6):1609-1620. PMID: 25668239.

View  

Seltmann et al. 2015. J Invest Dermatol. 135(6):1609-20. PMID: 25668239.

FACS IF

View  

Keratins Stabilize Hemidesmosomes through Regulation of β4-Integrin Turnover.

View  

Stawikowski et al. 2014. J Biol Chem. 289(31):21591-604. PMID: 24958723.

IF

View  

Glycosylation modulates melanoma cell α2β1 and α3β1 integrin interactions with type IV collagen.

View  

Pytlík et al. 2009. Biomaterials. 30(20):3415-27. PMID: 19362364.

FACS

View  

The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.

View  

Turner et al. 2006. Circulation. 114(8):820-9. PMID: 16908762.

View  

Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study.

View  

Berdichevsky et al. 1994. J Cell Sci. 107 ( Pt 12):3557-68. PMID: 7535787.

View  

Branching morphogenesis of human mammary epithelial cells in collagen gels.

View  

Add a reference

Inventor Information