Anti-Integrin a2 [HAS-3]
Invented by Prof Fiona Watt from King's College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (10)
- Inventor Info
Info
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| Catalogue Number | 151104 |
| Applications | FACS IHC IF IP |
| Antigen/Gene or Protein Targets | Integrin a2 (CD49b) |
| Synonyms | Integrin Subunit Alpha 2; Alpha 2 Subunit Of VLA-2 Receptor; Platelet Membrane Glycoprotein Ia; CD49 Antigen-Like Family Member B; Collagen Receptor; CD49B; GPIa; Very Late Activation Protein 2 Receptor, Alpha-2 Subunit; Human Platelet Alloantigen System 5; Platelet Glycoprotein GPIa; Platelet Antigen Br; VLA-2 Subunit Alpha; CD49b Antigen; HPA-5; VLA-2; VLAA2; BR |
| Reactivity | Human |
| Relevance |
Monoclonal antibody which co-precipitates a2b1 integrin from keratinocytes and inhibits a2b1 motility in collagen. Background and Research Application Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin a2 associates with integrin ß1 (CD29) to form the heterodimer integrin a2ß1, which is one of the most abundant keratinocyte integrins, is found distributed in the basal layer of epidermis and binds collagen. Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2. HAS-3 co-precipitates integrin a2ß1 from keratinocytes and inhibits HGF-induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen. |
| Host | Mouse |
| Immunogen | Whole human keratinocytes. |
| Immunogen UniProt ID | P17301 |
| Subclass | IgG2a |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 10% FCS |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest HAS-3 did not inhibit adhesion to laminin, fibronectin and type IV collagen. In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and co-stimulates mast cell activation, triggering neutrophil influx. Concentration 1mg/ml as standard |
| Research Area | Adhesion, Cancer, Cell Signaling & Signal Transduction, Metabolism |
References: 10 entries
Activation of c-MET induces a stem-like phenotype in human prostate cancer.
Europe PMC ID: 22110593
van Leenders et al. 2011. PLoS One. 6(11):e26753. PMID: 22110593.
Teklemariam et al. 2011. Toxicon. 57(5):646-56. PMID: 21255601.
Functional analysis of a recombinant PIII-SVMP, GST-acocostatin; an apoptotic inducer of HUVEC and HeLa, but not SK-Mel-28 cells.
Europe PMC ID: 21255601
Singh et al. 2010. Am J Physiol Lung Cell Mol Physiol. 299(2):L169-83. PMID: 20435685.
Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways?
Europe PMC ID: 20435685
Alford et al. 1998. J Cell Sci. 111 ( Pt 4):521-32. PMID: 9443900.
Integrin-matrix interactions affect the form of the structures developing from human mammary epithelial cells in collagen or fibrin gels.
Europe PMC ID: 9443900
Tenchini et al. 1993. Cell Adhes Commun. 1(1):55-66. PMID: 7521749.
Evidence against a major role for integrins in calcium-dependent intercellular adhesion of epidermal keratinocytes.
Europe PMC ID: 7521749
Add a reference
References: 10 entries
Activation of c-MET induces a stem-like phenotype in human prostate cancer.
van Leenders et al. 2011. PLoS One. 6(11):e26753. PMID: 22110593.
Teklemariam et al. 2011. Toxicon. 57(5):646-56. PMID: 21255601.
Functional analysis of a recombinant PIII-SVMP, GST-acocostatin; an apoptotic inducer of HUVEC and HeLa, but not SK-Mel-28 cells.
Singh et al. 2010. Am J Physiol Lung Cell Mol Physiol. 299(2):L169-83. PMID: 20435685.
Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways?
Alford et al. 1998. J Cell Sci. 111 ( Pt 4):521-32. PMID: 9443900.
Integrin-matrix interactions affect the form of the structures developing from human mammary epithelial cells in collagen or fibrin gels.
Tenchini et al. 1993. Cell Adhes Commun. 1(1):55-66. PMID: 7521749.
Evidence against a major role for integrins in calcium-dependent intercellular adhesion of epidermal keratinocytes.
Add a reference
