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Monoclonal Antibodies

Anti-HPV18E6 [BF7]

Invented by Dr Lawrence Banks from International Centre For Genetic Engineering And Biotechnology (ICGEB)
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Cheng Y, Geng L, Zhao L, Zuo P, Wang J. Human papillomavirus E6-regulated microRNA-20b promotes invasion in cervical cancer by targeting tissue inhibitor of metalloproteinase 2. Mol Med Rep. 2017 Oct;16(4):5464-5470. doi: 10.3892/mmr.2017.7231. Epub 2017 Aug 11. PMID: 28849054.HPV and miR-20b regulated the expression of TIMP-2 protein, and miR-20b targeted it. (A) The predicted interaction site of miR-20b and candidate target gene TIMP-2 3′UTR. (B) qPCR analysis of TIMP-2 mRNA level in HeLa cells transfected with miR-106b inhibitor (anti-miR-106b) or miR-106b control inhibitor (anti-NC). Data are mean ± SEM (n=3). (C) Western blot analysis of TIMP-2 protein level in HeLa cells transfected with miR-106b inhibitor (anti-miR-106b) or miR-106b control inhibitor (anti-NC). (D) Luciferase assay of HeLa cells co-transfected with miR-20b mimics and pGL-3-TIMP-2 plasmid (miR-NC and miR-106b with TIMP-2-WT-3′UTR; miR-NC and miR-106b with TIMP-2-Mut-3′UTR) after 24 h. Data are mean ± SEM (n=3). **P<0.01. (E) TIMP-2 protein level after siRNA knockdown of HPV18 E6 protein and (F) quantification. Data are mean ± SEM (n=3). *P<0.05 vs. control, #P<0.05 vs. E6/GAPDH.
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Catalogue Number 151080
Applications ELISA RIA WB
Antigen/Gene or Protein Targets Human Papilloma Virus-18 early protein 6 (HPV18 E6)
Reactivity Virus
Relevance The human papilloma virus (HPV) family of DNA tumor viruses includes HPV-16 and HPV-18, which are associated with a large proportion of cervical cancer cases. HPV early proteins E6 and E7 are the major viral oncoproteins that regulate cell proliferation through the inactivation of p53 and Rb1 tumour suppressor proteins respectively. BF7 can be used for detection of HPV in cervical smears and biopsies and analysis of E6 expression in cell transformation studies.
Host Mouse
Immunogen Gel-purified HPV-18 E6-b galactosidase fusion protein.
Positive Control HPV infected tissue
Subclass IgG1
Molecular Weight (kDa) 16.5
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Reactivity: Herpes simplex virus (HSV)
Research Area Cancer, Virology

References

There are 3 reference entries for this reagent.

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References: 3 entries

Cheng et al. 2017. Mol Med Rep. 16(4):5464-5470. PMID: 28849054.

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Banks et al. 1987. J Gen Virol. 68 ( Pt 5):1351-9. PMID: 3033140.

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Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas.

Europe PMC ID: 3033140

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References: 3 entries

Cheng et al. 2017. Mol Med Rep. 16(4):5464-5470. PMID: 28849054.

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Banks et al. 1987. J Gen Virol. 68 ( Pt 5):1351-9. PMID: 3033140.

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Identification of human papillomavirus type 18 E6 polypeptide in cells derived from human cervical carcinomas.

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