Anti-BCRABL, Recombinant [7c6]
Invented at Absolute Antibody Queen Mary University of London
Anti-BCRABL [7c6]
Invented at Queen Mary University of London
- Datasheet
- References (7)
- Inventor Info
Info
| Catalogue Number | 151062 |
| Applications | IF IP WB |
| Antigen/Gene or Protein Targets | BCRABL |
| Reactivity | Human |
| Relevance |
Monoclonal antibody directed against BCR-ABL oncoprotein. Background and Research Application The BCR gene is involved in the 9:22 translocation that generates the Philadelphia chromosome both in chronic myeloid leukaemia (CML) and in a proportion of cases of acute lymphocytic leukaemia (ALL). A 5' bcr sequence becomes fused to an abl sequence (including tyrosine kinase domain sequences) from chromosome 9. This results in the production of a chimeric BCR-ABL oncoprotein with constitutively active tyrosine kinase activity. Anti-BCRABL antibody may be useful for studies of the oncogene and have potential diagnostic and prognostic applications, particularly within leukaemia. This antibody is specific for the SSINEEITPRRQS epitope of BCR (residues 686-696), and as such is able to recognise full-length BCR and the p210 form of the BCR-ABL fusion protein. |
| Host | Mouse |
| Immunogen | Bcr686 thyroglobulin conjugate |
| Immunogen UniProt ID | Q62199 |
| Subclass | IgG2a |
| Molecular Weight (kDa) | 210 |
| Myeloma Used | P3X63Ag8 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody can detect BCR-ABL protein and full length BCR. Concentration 1mg/ml as standard |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling |
References: 7 entries
Mallery et al. 2017. Cancer Prev Res (Phila). 10(1):76-88. PMID: 27756753.
Dhut et al. 1990. Leukemia. 4(11):745-50. PMID: 2232885.
BCR-ABL and BCR proteins: biochemical characterization and localization.
Europe PMC ID: 2232885
Dhut et al. 1988. Oncogene. 3(5):561-6. PMID: 3078961.
Identification of two normal bcr gene products in the cytoplasm.
Europe PMC ID: 3078961
Walker et al. 1987. Nature. 329(6142):851-3. PMID: 3478595.
Novel chimaeric protein expressed in Philadelphia positive acute lymphoblastic leukaemia.
Europe PMC ID: 3478595
Add a reference
References: 7 entries
Mallery et al. 2017. Cancer Prev Res (Phila). 10(1):76-88. PMID: 27756753.
Dhut et al. 1990. Leukemia. 4(11):745-50. PMID: 2232885.
BCR-ABL and BCR proteins: biochemical characterization and localization.
Dhut et al. 1988. Oncogene. 3(5):561-6. PMID: 3078961.
Identification of two normal bcr gene products in the cytoplasm.
Walker et al. 1987. Nature. 329(6142):851-3. PMID: 3478595.
Novel chimaeric protein expressed in Philadelphia positive acute lymphoblastic leukaemia.
Add a reference
