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Monoclonal Antibodies

Anti-CD8 [14]

Invented by Prof Nancy Hogg from Cancer Research UK London Research Institute: Lincoln's Inn Fields
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Xiao F, Ma L, Zhao M, Huang G, Mirenda V, Dorling A, Lechler R, Lombardi G. Ex vivo expanded human regulatory T cells delay islet allograft rejection via inhibiting islet-derived monocyte chemoattractant protein-1 production in CD34+ stem cells-reconstituted NOD-scid IL2rγnull mice. PLoS One. 2014 Mar 3;9(3):e90387. doi: 10.1371/journal.pone.0090387. Erratum in: PLoS One. 2014;9(5):e98722. PMID: 24594640; PMCID: PMC3940883. Infiltration of immune cells and C3 deposit in the grafted human islets. Tissue samples of islet allograft bearing kidney in hu-NSG mice were collected at the time of islet allograft rejection. Sections were stained with H & E (a–b, e–f and i) and antibodies to human antigens: insulin (c and g, brown), CD45 (d and h, green), CD4 (j, green), CD8 (k, red), CD11b (l, green), CD66b (m, red), and C3d (n, brown). Arrow indicates the kidney capsule or the area around islet grafts. Red square indicates the site of the infiltration. Scale bar: 100 µm.
Adapted from Hogg N, Stewart MP, Scarth SL, Newton R, Shaw JM, Law SK, Klein N. A novel leukocyte adhesion deficiency caused by expressed but nonfunctional beta2 integrins Mac-1 and LFA-1. J Clin Invest. 1999 Jan;103(1):97-106. doi: 10.1172/JCI3312. PMID: 9884339; PMCID: PMC407855. Flow cytometric analysis of patient and control T cells comparing membrane expression levels of LFA-1 and other T cell–specific membrane receptors. LAD, leukocyte adhesion deficiency; MFI, mean fluorescence intensity (units); MAB, monoclonal antibody
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Catalogue Number 151035
Applications ELISA FACS IHC IP
Antigen/Gene or Protein Targets CD8
Synonyms Leu2
Reactivity Human
Relevance CD8 is a T cell co-receptor that recognises, together with the T cell receptor, MHC class I molecules. CD8 is present on human suppressor / cytotoxic T cells, 30% of circulating T cells. Clone 14 is recommended for: the detection of immunoregulatory T cell subset imbalances in autoimmune disorders and immunodeficiency states, categorisation of T cell acute lymphoblastic leukaemia and lymphoblastic lymphoma in conjunction with other antigens and determination of CD4/CD8 ratios in immune deficiencies, rheumatoid arthritis and multiple sclerosis.
Host Mouse
Immunogen Fibronectin-purified human monocytes
Subclass IgG1
Recommended Growing Conditions DMEM + 5% FCS
Notes Recommended for T-cell enrichment by cell sorting or panning.

Suitability for western blotting not known; to be confirmed - therefore WB not listed as a definite application.
Research Area Cancer, Immunology, Stem Cell Biology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Hogg et al. 1999. J Clin Invest. 103(1):97-106. PMID: 9884339.

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A novel leukocyte adhesion deficiency caused by expressed but nonfunctional beta2 integrins Mac-1 and LFA-1.

Europe PMC ID: 9884339

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References: 2 entries

Hogg et al. 1999. J Clin Invest. 103(1):97-106. PMID: 9884339.

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A novel leukocyte adhesion deficiency caused by expressed but nonfunctional beta2 integrins Mac-1 and LFA-1.

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